AMPK-Nrf2 Signaling Pathway in Phrenic Motoneurons following Cervical Spinal Cord Injury.
AMPK
Nrf2
antioxidant
neuroinflammation
phrenic motoneuron
rat
spinal cord injury
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
26 Aug 2022
26 Aug 2022
Historique:
received:
28
07
2022
revised:
24
08
2022
accepted:
25
08
2022
entrez:
23
9
2022
pubmed:
24
9
2022
medline:
24
9
2022
Statut:
epublish
Résumé
High spinal cord injuries (SCI) induce the deafferentation of phrenic motoneurons, leading to permanent diaphragm paralysis. This involves secondary injury associated with pathologic and inflammatory processes at the site of injury, and at the level of phrenic motoneurons. In the present study, we evaluated the antioxidant response in phrenic motoneurons involving the AMPK-Nrf2 signaling pathway following C2 spinal cord lateral hemi-section in rats. We showed that there is an abrupt reduction in the expression of phosphorylated AMPK and Nrf2 at one hour post-injury in phrenic motoneurons. A rebound is then observed at one day post-injury, reflecting a return to homeostasis condition. In the total spinal cord around phrenic motoneurons, the increase in phosphorylated AMPK and Nrf2 occurred at three days post-injury, showing the differential antioxidant response between phrenic motoneurons and other cell types. Taken together, our results display the implication of the AMPK-Nrf2 signaling pathway in phrenic motoneurons' response to oxidative stress following high SCI. Harnessing this AMPK-Nrf2 signaling pathway could improve the antioxidant response and help in spinal rewiring to these deafferented phrenic motoneurons to improve diaphragm activity in patients suffering high SCI.
Identifiants
pubmed: 36139739
pii: antiox11091665
doi: 10.3390/antiox11091665
pmc: PMC9495920
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Chancellerie des Universités de Paris
ID : (Legs Poix)
Organisme : Fondation de France
ID : Fondation Médisite
Organisme : INSERM
ID : U1179
Organisme : Université Saint Quentin en Yvelines
ID : AAPSI
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