Myosins and MyomiR Network in Patients with Obstructive Hypertrophic Cardiomyopathy.

MyomiR (myosin-encoded miRs) Polypyrimidine Tract Binding Protein 3 (PTBP3) SRY-Box Transcription Factor 6 (SOX6) cardiomyocytes hypertrophic cardiomyopathy (HCM) miR-499 microRNA (miRs) myosin heavy chain (isoform: MyHC, gene: MYH)

Journal

Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304

Informations de publication

Date de publication:
03 Sep 2022
Historique:
received: 20 06 2022
revised: 30 08 2022
accepted: 01 09 2022
entrez: 23 9 2022
pubmed: 24 9 2022
medline: 24 9 2022
Statut: epublish

Résumé

Hypertrophic cardiomyopathy (HCM) is the most common genetic cardiomyopathy. The molecular mechanisms determining HCM phenotypes are incompletely understood. Myocardial biopsies were obtained from a group of patients with obstructive HCM (n = 23) selected for surgical myectomy and from 9 unused donor hearts (controls). A subset of tissue-abundant myectomy samples from HCM (n = 10) and controls (n = 6) was submitted to laser-capture microdissection to isolate cardiomyocytes. We investigated the relationship among clinical phenotype, cardiac myosin proteins (MyHC6, MyHC7, and MyHC7b) measured by optimized label-free mass spectrometry, the relative genes (

Identifiants

pubmed: 36140281
pii: biomedicines10092180
doi: 10.3390/biomedicines10092180
pmc: PMC9496008
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Ministero della Salute
ID : NET-2011-02347173

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Chiara Foglieni (C)

Cardiovascular Research Center, IRCCS San Raffaele Hospital, 58, 20132 Milan, Italy.

Maria Lombardi (M)

Cardiovascular Research Center, IRCCS San Raffaele Hospital, 58, 20132 Milan, Italy.

Davide Lazzeroni (D)

Cardiovascular Research Center, IRCCS San Raffaele Hospital, 58, 20132 Milan, Italy.

Riccardo Zerboni (R)

Cardiovascular Research Center, IRCCS San Raffaele Hospital, 58, 20132 Milan, Italy.

Edoardo Lazzarini (E)

Laboratory for Cardiovascular Theranostics, Cardiocentro Ticino Institute, Ente Ospedaliero Cantonale and Faculty of Biomedical Sciences, Università Svizzera Italiana, 6900 Lugano, Switzerland.

Gloria Bertoli (G)

Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 20054 Milan, Italy.

Annalinda Pisano (A)

Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Policlinico Umberto I, 00185 Rome, Italy.

Francesca Girolami (F)

Cardiology Unit, Meyer Children's Hospital, 50139 Florence, Italy.

Annapaola Andolfo (A)

ProMeFa, Proteomics and Metabolomics Facility, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.

Cinzia Magagnotti (C)

ProMeFa, Proteomics and Metabolomics Facility, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.

Giovanni Peretto (G)

IRCCS San Raffaele Scientific Institute, Vita-Salute University, 20132 Milan, Italy.

Carmem L Sartorio (CL)

Cardiovascular Research Center, IRCCS San Raffaele Hospital, 58, 20132 Milan, Italy.

Iacopo Olivotto (I)

Cardiomyopathy Unit, Careggi University Hospital, 50134 Florence, Italy.

Giovanni La Canna (G)

IRCCS Humanitas Clinical and Research Institute, 20089 Rozzano, Italy.

Ottavio Alfieri (O)

Cardiac Surgery Unit, IRCCS San Raffaele Scientific Institute, 20132 Milan, Italy.

Ornella E Rimoldi (OE)

Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), 20054 Milan, Italy.

Lucio Barile (L)

Laboratory for Cardiovascular Theranostics, Cardiocentro Ticino Institute, Ente Ospedaliero Cantonale and Faculty of Biomedical Sciences, Università Svizzera Italiana, 6900 Lugano, Switzerland.

Giulia d'Amati (G)

Department of Radiological, Oncological and Pathological Sciences, Sapienza University of Rome, Policlinico Umberto I, 00185 Rome, Italy.

Paolo G Camici (PG)

Cardiovascular Research Center, IRCCS San Raffaele Hospital, 58, 20132 Milan, Italy.
IRCCS San Raffaele Scientific Institute, Vita-Salute University, 20132 Milan, Italy.

Classifications MeSH