Impact of Host Telomere Length on HHV-6 Integration.
HHV-6
TZAP
human herpesvirus 6
integration
telomere length
telomeres
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
24 08 2022
24 08 2022
Historique:
received:
05
07
2022
revised:
15
08
2022
accepted:
21
08
2022
entrez:
23
9
2022
pubmed:
24
9
2022
medline:
28
9
2022
Statut:
epublish
Résumé
Human herpesvirus 6A and 6B are two closely related viruses that infect almost all humans. In contrast to most herpesviruses, HHV-6A/B can integrate their genomes into the telomeres during the infection process. Both viruses can also integrate in germ cells and subsequently be inherited in children. How HHV-6A/B integrate into host telomeres and the consequences of this remain a subject of active research. Here, we developed a method to measure telomere length by quantitative fluorescence in situ hybridization, confocal microscopy, and computational processing. This method was validated using a panel of HeLa cells having short or long telomeres. These cell lines were infected with HHV-6A, revealing that the virus could efficiently integrate into telomeres independent of their length. Furthermore, we assessed the telomere lengths after HHV-6A integration and found that the virus-containing telomeres display a variety of lengths, suggesting that either telomere length is restored after integration or telomeres are not shortened by integration. Our results highlight new aspects of HHV-6A/B biology and the role of telomere length on virus integration.
Identifiants
pubmed: 36146670
pii: v14091864
doi: 10.3390/v14091864
pmc: PMC9505050
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : Stg 677673
Pays : International
Références
Radiat Res. 2001 Jan;155(1 Pt 2):188-193
pubmed: 11121233
Front Microbiol. 2019 Jun 26;10:1408
pubmed: 31293546
Apoptosis. 2001 Dec;6(6):447-52
pubmed: 11595834
Cell. 1989 Nov 3;59(3):521-9
pubmed: 2805070
Cell. 1998 Feb 6;92(3):401-13
pubmed: 9476899
Lancet. 2003 Sep 20;362(9388):983-8
pubmed: 14511933
Nature. 1990 May 31;345(6274):458-60
pubmed: 2342578
J Med Virol. 2009 Feb;81(2):258-63
pubmed: 19107978
Br J Haematol. 2013 May;161(4):594-5
pubmed: 23406281
Lancet. 1988 May 14;1(8594):1065-7
pubmed: 2896909
Nature. 1993 Oct 7;365(6446):566-8
pubmed: 7692304
Methods Mol Biol. 2013;1064:141-52
pubmed: 23996255
J Gen Virol. 2016 Aug;97(8):1899-1903
pubmed: 27170009
Sci Rep. 2014 Apr 02;4:4559
pubmed: 24691081
Clin Microbiol Rev. 2015 Apr;28(2):313-35
pubmed: 25762531
Virology. 2013 Jul 20;442(1):3-11
pubmed: 23648233
Hum Mol Genet. 1996 May;5(5):685-91
pubmed: 8733138
Virology. 1991 Oct;184(2):545-52
pubmed: 1653487
J Exp Med. 2011 Mar 14;208(3):605-15
pubmed: 21383055
Nat Protoc. 2010 Sep;5(9):1596-607
pubmed: 21085125
J Med Virol. 2010 Oct;82(10):1669-78
pubmed: 20827763
J Immunol. 1995 Oct 15;155(8):3711-5
pubmed: 7561072
J Gen Virol. 1995 Feb;76 ( Pt 2):451-8
pubmed: 7844567
Biochemistry (Mosc). 2010 Dec;75(13):1563-83
pubmed: 21417995
Nat Microbiol. 2020 Jul;5(7):901-908
pubmed: 32367053
EMBO Rep. 2017 Jun;18(6):929-946
pubmed: 28500257
Nucleic Acids Res. 2014 Jan;42(1):315-27
pubmed: 24057213
Dev Genet. 1996;18(2):173-9
pubmed: 8934879
Ann Anat. 2010 Sep 20;192(5):284-91
pubmed: 20732797
J Infect Dis. 1994 Apr;169(4):943-4
pubmed: 8133119
Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):8058-63
pubmed: 26080419
Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5563-8
pubmed: 20212114
Curr Opin Virol. 2014 Dec;9:111-8
pubmed: 25462442
Exp Cell Res. 1965 Mar;37:614-36
pubmed: 14315085
Viruses. 2017 Jun 26;9(7):
pubmed: 28672870
Science. 2017 Feb 10;355(6325):638-641
pubmed: 28082411
J Med Virol. 2008 Nov;80(11):1952-8
pubmed: 18814270
PLoS Pathog. 2020 Apr 22;16(4):e1008496
pubmed: 32320442
Blood. 1999 Sep 1;94(5):1545-9
pubmed: 10477678
Arterioscler Thromb Vasc Biol. 2003 May 1;23(5):842-6
pubmed: 12649083
J Virol. 1999 Oct;73(10):8040-52
pubmed: 10482553
Clin Microbiol Rev. 1997 Jul;10(3):521-67
pubmed: 9227865
Antiviral Res. 2020 Apr;176:104720
pubmed: 32044155
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6
pubmed: 3413114
Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5300-5
pubmed: 17369361
Nucleic Acids Res. 2015 Jul 13;43(12):6084-98
pubmed: 25999342
Nature. 1999 Jun 24;399(6738):806-9
pubmed: 10391249
J Biol Chem. 2002 May 31;277(22):19679-87
pubmed: 11901159
Lancet. 2005 Aug 20-26;366(9486):662-4
pubmed: 16112303
J Virol. 2017 Jun 26;91(14):
pubmed: 28468878
Nucleic Acids Res. 2018 May 18;46(9):4487-4504
pubmed: 29490055
Nurs Res. 2014 Jul-Aug;63(4):289-99
pubmed: 24977726
Clin Infect Dis. 2014 Aug 15;59(4):545-8
pubmed: 24803376
Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31410-31416
pubmed: 33229517
Curr Opin Cell Biol. 1991 Jun;3(3):444-51
pubmed: 1892656
Cell. 1999 May 14;97(4):503-14
pubmed: 10338214
Annu Rev Virol. 2014 Nov;1(1):215-35
pubmed: 26958721
J Med Virol. 2004 Jul;73(3):465-73
pubmed: 15170644
Mol Biol Evol. 2021 Jan 4;38(1):96-107
pubmed: 32722766
Clin Microbiol Rev. 2005 Jan;18(1):217-45
pubmed: 15653828
PLoS Pathog. 2016 May 31;12(5):e1005666
pubmed: 27244446
J Med Virol. 2010 Oct;82(10):1684-8
pubmed: 20827765
Bio Protoc. 2015 Nov 20;5(22):
pubmed: 27500189
Science. 2009 Nov 13;326(5955):948-52
pubmed: 19965504
Exp Cell Res. 1961 Dec;25:585-621
pubmed: 13905658
Am J Transplant. 2009 Jul;9(7):1690-7
pubmed: 19519818
J Virol. 1994 May;68(5):3007-14
pubmed: 8151770
Viruses. 2018 Nov 21;10(11):
pubmed: 30469324