CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected Cells.
Ebola virus
actin
assembly
egress
host interaction
trafficking
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
28 08 2022
28 08 2022
Historique:
received:
03
08
2022
revised:
24
08
2022
accepted:
26
08
2022
entrez:
23
9
2022
pubmed:
24
9
2022
medline:
28
9
2022
Statut:
epublish
Résumé
The replication of Ebola virus (EBOV) is dependent upon actin functionality, especially at cell entry through macropinocytosis and at release of virus from cells. Previously, major actin-regulatory factors involved in actin nucleation, such as Rac1 and Arp2/3, were shown important in both steps. However, downstream of nucleation, many other cell factors are needed to control actin dynamics. How these regulate EBOV infection remains largely unclear. Here, we identified the actin-regulating protein, CAPG, as important for EBOV replication. Notably, knockdown of CAPG specifically inhibited viral infectivity and yield of infectious particles. Cell-based mechanistic analysis revealed a requirement of CAPG for virus production from infected cells. Proximity ligation and split-green fluorescent protein reconstitution assays revealed strong association of CAPG with VP40 that was mediated through the S1 domain of CAPG. Overall, CAPG is a novel host factor regulating EBOV infection through connecting actin filament stabilization to viral egress from cells.
Identifiants
pubmed: 36146710
pii: v14091903
doi: 10.3390/v14091903
pmc: PMC9505868
pii:
doi:
Substances chimiques
Actins
0
Microfilament Proteins
0
Nuclear Proteins
0
Viral Matrix Proteins
0
Green Fluorescent Proteins
147336-22-9
CAPG protein, human
148412-71-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : P01 AI120943
Pays : United States
Organisme : NIAID NIH HHS
ID : UC7 AI095321
Pays : United States
Organisme : NIAID NIH HHS
ID : P01AI120943
Pays : United States
Organisme : NIAID NIH HHS
ID : UC7AI095321
Pays : United States
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