Single-Cell Transcriptomics Analysis of the Pathogenesis of Tendon Injury.
Journal
Oxidative medicine and cellular longevity
ISSN: 1942-0994
Titre abrégé: Oxid Med Cell Longev
Pays: United States
ID NLM: 101479826
Informations de publication
Date de publication:
2022
2022
Historique:
received:
12
07
2022
revised:
08
08
2022
accepted:
13
08
2022
entrez:
23
9
2022
pubmed:
24
9
2022
medline:
28
9
2022
Statut:
epublish
Résumé
Tendon injury repair has been a clinical challenge, and little is known about tendon healing scar generation, repair, and regeneration mechanisms. To explore the cellular composition of tendon tissue and analyze cell populations and signaling pathways associated with tendon repair, in this paper, single-cell sequencing data was used for data mining and seven cell subsets were annotated in the tendon tissue, including fibroblasts, tenocytes, smooth muscle cells, endothelial cells, macrophages, T cells, and plasma cells. According to cell group interaction network analysis, pattern 4 composed of macrophages was an important communication pattern in tendon injury. Furthermore, the heterogeneity of M1 macrophages in tendons, the correlation of KEGG enriched pathway with inflammatory response, and the core regulatory role of the transcription factor NFKB and REL were observed; in addition, the heterogeneity of T cell isoforms in tendons was found and indicated that different isotypes of T cells involve in different roles of tendon injury and repair. This study demonstrated the heterogeneity of M1 macrophages and T cells in the tendon tissue, being involved in different physiological processes such as tendon injury and healing, providing new thinking insights and basis for subsequent clinical treatment of tendon injury.
Identifiants
pubmed: 36148412
doi: 10.1155/2022/7887782
pmc: PMC9489412
doi:
Substances chimiques
Protein Isoforms
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7887782Informations de copyright
Copyright © 2022 Haibo Zhao et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
Références
Tissue Barriers. 2020;8(1):1695491
pubmed: 31818195
Int J Mol Sci. 2020 Sep 14;21(18):
pubmed: 32937830
Nat Rev Rheumatol. 2015 Apr;11(4):223-33
pubmed: 25734975
J Appl Physiol (1985). 2020 Mar 1;128(3):473-482
pubmed: 31944888
Bone Res. 2015 Oct 06;3:15015
pubmed: 26509098
Adv Drug Deliv Rev. 2015 Apr;84:222-39
pubmed: 25446135
J Musculoskelet Neuronal Interact. 2006 Apr-Jun;6(2):181-90
pubmed: 16849830
Rheumatology (Oxford). 2006 May;45(5):508-21
pubmed: 16490749
Front Vet Sci. 2015 Nov 16;2:55
pubmed: 26664982
J Hand Surg Am. 2004 Jul;29(4):551-63
pubmed: 15249076
Mol Aspects Med. 2018 Feb;59:36-46
pubmed: 28754496
Sci Adv. 2016 Nov 18;2(11):e1600874
pubmed: 28138519
Sports Med. 2003;33(5):381-94
pubmed: 12696985
J Orthop Sci. 2002;7(4):508-14
pubmed: 12181670
Nat Commun. 2021 Feb 17;12(1):1088
pubmed: 33597522
Annu Rev Biomed Eng. 2012;14:47-71
pubmed: 22809137
Front Med (Lausanne). 2021 Jul 28;8:664909
pubmed: 34395467