Microfocus computed tomography for fetal postmortem imaging: an overview.
Anatomy
Autopsy
Diffusible iodine-based contrast-enhanced computed tomography
Fetus
Human
Intrauterine fetal demise
Microfocus computed tomography
Postmortem
Stillbirth
Journal
Pediatric radiology
ISSN: 1432-1998
Titre abrégé: Pediatr Radiol
Pays: Germany
ID NLM: 0365332
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
received:
26
05
2022
accepted:
06
09
2022
revised:
18
07
2022
pubmed:
29
9
2022
medline:
23
3
2023
entrez:
28
9
2022
Statut:
ppublish
Résumé
Over the last few years, fetal postmortem microfocus computed tomography (micro-CT) imaging has increased in popularity for both diagnostic and research purposes. Micro-CT imaging could be a substitute for autopsy, particularly in very early gestation fetuses for whom autopsy can be technically challenging and is often unaccepted by parents. This article provides an overview of the latest research in fetal postmortem micro-CT imaging with a focus on diagnostic accuracy, endovascular staining approaches, placental studies and the reversibility of staining. It also discusses new methods that could prove helpful for micro-CT of larger fetuses. While more research is needed, contrast-enhanced micro-CT has the potential to become a suitable alternative to fetal autopsy. Further research using this novel imaging tool could yield wider applications, such as its practise in imaging rare museum specimens.
Identifiants
pubmed: 36169668
doi: 10.1007/s00247-022-05517-1
pii: 10.1007/s00247-022-05517-1
pmc: PMC10027643
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
632-639Subventions
Organisme : Medical Research Council
ID : MR/R002118/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
Références
Lewis C, Simcock IC, Arthurs OJ (2021) Improving uptake of perinatal autopsy. Curr Opin Obstet Gynaecol 33:129–134
doi: 10.1097/GCO.0000000000000691
Ernst LM (2015) A pathologist’s perspective on the perinatal autopsy. Semin Perinatol 39:55–63
doi: 10.1053/j.semperi.2014.10.008
pubmed: 25511296
Kamphuis-Van Ulzen K, Koopmanschap DHJLM, Marcelis CLM et al (2016) When is a post-mortem skeletal survey of the fetus indicated, and when not? J Matern Neonatal Med 29:991–997
doi: 10.3109/14767058.2015.1029913
Bourlière-Najean B, Russel AS, Panuel M et al (2003) Value of fetal skeletal radiographs in the diagnosis of fetal death. Eur Radiol 13:1046–1049
doi: 10.1007/s00330-002-1474-3
pubmed: 12695826
Dawood Y, Buijtendijk MFJ, Shah H et al (2022) Imaging fetal anatomy. Semin Cell Dev Biol. https://doi.org/10.1016/j.semcdb.2022.02.023
doi: 10.1016/j.semcdb.2022.02.023
pubmed: 35282997
Gignac PM, Kley NJ, Clarke JA (2016) Diffusible iodine-based contrast-enhanced computed tomography (diceCT): an emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues. J Anat 228:889–909
doi: 10.1111/joa.12449
pubmed: 26970556
pmcid: 5341577
Lewis C, Hill M, Arthurs OJ et al (2018) Factors affecting uptake of postmortem examination in the prenatal, perinatal and paediatric setting. BJOG 125:172–181
doi: 10.1111/1471-0528.14600
pubmed: 28190300
Dawood Y, Strijkers GJ, Limpens J et al (2020) Novel imaging techniques to study postmortem human fetal anatomy: a systematic review on microfocus-CT and ultra-high-field MRI. Eur Radiol 30:2280–2292
doi: 10.1007/s00330-019-06543-8
pubmed: 31834508
Simcock IC, Shelmerdine SC, Hutchinson JC et al (2021) Human fetal whole-body postmortem microfocus computed tomographic imaging. Nat Protoc 16:2594–2614
doi: 10.1038/s41596-021-00512-6
pubmed: 33854254
Dawood Y, Hagoort J, Siadari BA et al (2021) Reducing soft-tissue shrinkage artefacts caused by staining with Lugol’s solution. Sci Rep 11:19781
doi: 10.1038/s41598-021-99202-2
pubmed: 34611247
pmcid: 8492742
Shelmerdine SC, Hutchinson JC, Arthurs OJ, Sebire NJ (2020) Latest developments in post-mortem foetal imaging. Prenat Diagn 40:28–37
doi: 10.1002/pd.5562
pubmed: 31525275
Shelmerdine SC, Simcock IC, Hutchinson JC et al (2021) Postmortem microfocus computed tomography for noninvasive autopsies: experience in >250 human fetuses. Am J Obstet Gynecol 224:103e1–103e15
Sandrini C, Boito S, Lombardi CM, Lombardi S (2021) Postmortem micro-CT of human fetal heart — a systematic literature review. J Clin Med 10:4726
doi: 10.3390/jcm10204726
pubmed: 34682849
pmcid: 8539069
Hutchinson JC, Arthurs OJ, Ashworth MT et al (2016) Clinical utility of postmortem microcomputed tomography of the fetal heart: diagnostic imaging vs. macroscopic dissection. Ultrasound Obstet Gynecol 47:58–64
doi: 10.1002/uog.15764
pubmed: 26415141
Prahlow JA, Ross KF, Salzberger L et al (1998) Immersion technique for brain removal in perinatal autopsies. J Forensic Sci 43:1056–1060
doi: 10.1520/JFS14357J
pubmed: 9729825
Sandaite I, Lombardi C, Cook AC et al (2020) Micro-computed tomography of isolated fetal hearts following termination of pregnancy: a feasibility study at 8 to 12 weeks’ gestation. Prenat Diagn 40:984–990
doi: 10.1002/pd.5719
pubmed: 32333804
Simcock IC, Shelmerdine SC, Langan D et al (2021) Micro-CT yields high image quality in human fetal post-mortem imaging despite maceration. BMC Med Imaging 21:128
doi: 10.1186/s12880-021-00658-5
pubmed: 34429085
pmcid: 8383392
Lupariello F, Genova T, Mussano F et al (2021) Micro-CT processing’s effects on microscopic appearance of human fetal cardiac samples. Leg Med 53:101934
doi: 10.1016/j.legalmed.2021.101934
Metscher BD (2009) MicroCT for comparative morphology: simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiol 9:11
doi: 10.1186/1472-6793-9-11
pubmed: 19545439
pmcid: 2717911
Metscher BD (2009) MicroCT for developmental biology: a versatile tool for high-contrast 3D imaging at histological resolutions. Dev Dyn 238:632–640
doi: 10.1002/dvdy.21857
pubmed: 19235724
Dawood Y, Honhoff C, van der Post AS et al (2021) Comparison of postmortem whole-body contrast-enhanced microfocus computed tomography and high field magnetic resonance imaging of human fetuses. Ultrasound Obstet Gynecol. https://doi.org/10.1002/uog.24827
doi: 10.1002/uog.24827
Lombardi CM, Zambelli V, Botta G (2014) Postmortem microcomputed tomography (micro-CT) of small fetuses and hearts. Ultrasound Obstet Gynecol 44:600–609
doi: 10.1002/uog.13330
pubmed: 24585450
Zhou YQ, Davidson L, Henkelman RM et al (2004) Ultrasound-guided left-ventricular catheterization: a novel method of whole mouse perfusion for microimaging. Lab Invest 84:385–389
doi: 10.1038/labinvest.3700038
pubmed: 14704721
Dunmore-Buyze PJ, Tate E, Xiang FL et al (2014) Three-dimensional imaging of the mouse heart and vasculature using micro-CT and whole-body perfusion of iodine or phosphotungstic acid. Contrast Media Mol Imaging 9:383–390
doi: 10.1002/cmmi.1588
pubmed: 24764151
Aydin E, Levy B, Oria M et al (2019) Optimization of pulmonary vasculature tridimensional phenotyping in the rat fetus. Sci Rep 9:1244
doi: 10.1038/s41598-018-37906-8
pubmed: 30718645
pmcid: 6362188
Schweitzer W, Koller B, Haemmerle S et al (2022) Fetal and newborn angiography using micro-CT and electric immersion pump — updated technical note. Abstract, International Society of Forensic Radiology and Imaging conference, Japan
Aughwane R, Schaaf C, Hutchinson JC et al (2019) Micro-CT and histological investigation of the spatial pattern of feto-placental vascular density. Placenta 88:36–43
doi: 10.1016/j.placenta.2019.09.014
pubmed: 31670095
pmcid: 6892277
James JL, Tongpob Y, Srinivasan V et al (2021) Three-dimensional visualisation of the feto-placental vasculature in humans and rodents. Placenta 114:8–13
doi: 10.1016/j.placenta.2021.08.049
pubmed: 34418753
Castro PT, Matos APP, Aranda OL et al (2019) Tuboperitoneal fistula, ectopic pregnancy, and remnants of fallopian tube: a confocal microtomography analysis and 3D reconstruction of human fallopian tube pathologies. J Matern Fetal Neonatal Med 32:3082–3087
doi: 10.1080/14767058.2018.1455181
pubmed: 29558232
FlowTek Inc. About Microfil. https://www.flowtech-inc.com/about/ . Accessed 25 Apr 2022
Lanzetti A, Ekdale EG (2021) Enhancing CT imaging: a safe protocol to stain and de-stain rare fetal museum specimens using diffusible iodine-based staining (diceCT). J Anat 239:228–241
doi: 10.1111/joa.13410
pubmed: 33665841