Cell-type identity of the avian utricle.

CP: Cell biology CP: Neuroscience extrastriola hair cell hair cell production inner ear striola supporting cell turnover vestibular apparatus

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
27 09 2022
Historique:
received: 08 04 2021
revised: 18 07 2022
accepted: 08 09 2022
entrez: 28 9 2022
pubmed: 29 9 2022
medline: 1 10 2022
Statut: ppublish

Résumé

The avian utricle, a vestibular organ of the inner ear, displays turnover of sensory hair cells throughout life. This is in sharp contrast to the mammalian utricle, which shows limited regenerative capacity. Here, we use single-cell RNA sequencing to identify distinct marker genes for the different sensory hair cell subtypes of the chicken utricle, which we validated in situ. We provide markers for spatially distinct supporting cell populations and identify two transitional cell populations of dedifferentiating supporting cells and developing hair cells. Trajectory reconstruction resulted in an inventory of gene expression dynamics of natural hair cell generation in the avian utricle.

Identifiants

pubmed: 36170825
pii: S2211-1247(22)01273-6
doi: 10.1016/j.celrep.2022.111432
pmc: PMC9588199
mid: NIHMS1839197
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

111432

Subventions

Organisme : NIDCD NIH HHS
ID : R01 DC019619
Pays : United States
Organisme : NIH HHS
ID : S10 OD018220
Pays : United States
Organisme : NIH HHS
ID : S10 OD021763
Pays : United States

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests S.H. is a paid consultant of Pipeline Therapeutics and Lineage Cell Therapeutics.

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Auteurs

Mirko Scheibinger (M)

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: mscheib@stanford.edu.

Amanda Janesick (A)

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

Nesrine Benkafadar (N)

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

Daniel C Ellwanger (DC)

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

Taha A Jan (TA)

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA.

Stefan Heller (S)

Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: hellers@stanford.edu.

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