Development of non-adherent cell-enclosing domes with enzymatically cross-linked hydrogel shell.

Humans Alginates / metabolism Horseradish Peroxidase Hydrogels Mitomycin
Cell Dome K562 cell alginate microcapsule microdome non-adherent cell

Journal

Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964

Informations de publication

Date de publication:
27 Oct 2022
Historique:
received: 07 06 2022
accepted: 28 09 2022
pubmed: 29 9 2022
medline: 29 10 2022
entrez: 28 9 2022
Statut: epublish

Résumé

Non-adherent cells, such as hematopoietic cells and lymphocytes, are important research subjects in medical and biological fields. Therefore, a system that enables the handling of non-adherent cells in solutions in the same manner as that of adhering cells during medium exchange, exposure to chemicals, washing, and staining in imaging applications would be useful. Here, we report a 'Cell Dome' platform in which non-adherent cells can be enclosed and grown in the cavities of about 1 mm diameter and 270

Identifiants

DOI: 10.1088/1758-5090/ac95ce PMID: 36170845
pubmed: 36170845
doi: 10.1088/1758-5090/ac95ce
doi:

Substances chimiques

Alginates 0
Horseradish Peroxidase EC 1.11.1.-
Hydrogels 0
Mitomycin 50SG953SK6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022 IOP Publishing Ltd.

Auteurs

Ryotaro Kazama (R)

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Ryuta Sato (R)

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Hiroyuki Fujiwara (H)

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Yanfei Qu (Y)

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Masaki Nakahata (M)

Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.

Masaru Kojima (M)

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.

Satoshi Fujita (S)

AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Suita, Osaka 565-0871, Japan.

Shinji Sakai (S)

Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
ORCID: 0000-0002-1041-4798

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Development of non-adherent cell-enclosing...
questionsmedicales.fr › Glucides › Polyosides › Alginates › Development of non-adherent cell-enclosing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Peroxidases › Horseradish peroxidase › Development of non-adherent cell-enclosing...
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Gels › Hydrogels › Development of non-adherent cell-enclosing...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Indolequinones › Mitomycines › Mitomycine › Development of non-adherent cell-enclosing...