Pre-validation of a reporter gene assay for oxidative stress for the rapid screening of nanobiomaterials.
Nrf2
interlaboratory validation
nanomaterial
nanotoxicology
oxidative stress
Journal
Frontiers in toxicology
ISSN: 2673-3080
Titre abrégé: Front Toxicol
Pays: Switzerland
ID NLM: 101777990
Informations de publication
Date de publication:
2022
2022
Historique:
received:
21
06
2022
accepted:
09
08
2022
entrez:
29
9
2022
pubmed:
30
9
2022
medline:
30
9
2022
Statut:
epublish
Résumé
Engineered nanomaterials have been found to induce oxidative stress. Cellular oxidative stress, in turn, can result in the induction of antioxidant and detoxification enzymes which are controlled by the nuclear erythroid 2-related factor 2 (NRF2) transcription factor. Here, we present the results of a pre-validation study which was conducted within the frame of BIORIMA ("biomaterial risk management") an EU-funded research and innovation project. For this we used an NRF2 specific chemically activated luciferase expression reporter gene assay derived from the human U2OS osteosarcoma cell line to screen for the induction of the NRF2 mediated gene expression following exposure to biomedically relevant nanobiomaterials. Specifically, we investigated Fe
Identifiants
pubmed: 36171865
doi: 10.3389/ftox.2022.974429
pii: 974429
pmc: PMC9511406
doi:
Types de publication
Journal Article
Langues
eng
Pagination
974429Informations de copyright
Copyright © 2022 Martin, de Haan, Miro Estruch, Eder, Marzi, Schnekenburger, Blosi, Costa, Antonello, Bergamaschi, Riganti, Beal, Carrière, Taché, Hutchison, Malone, Young, Campagnolo, La Civita, Pietroiusti, Devineau, Baeza, Boland, Zong, Ichihara, Fadeel and Bouwmeester.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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