P2X7 is expressed on human innate-like T lymphocytes and mediates susceptibility to ATP-induced cell death.
ATP
P2X7
cell death
innate-like T cells
nanobody
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
revised:
17
08
2022
received:
07
04
2022
accepted:
26
09
2022
pubmed:
1
10
2022
medline:
8
11
2022
entrez:
30
9
2022
Statut:
ppublish
Résumé
Extracellular ATP activates the P2X7 receptor, leading to inflammasome activation and release of pro-inflammatory cytokines in monocytes. However, a detailed analysis of P2X7 receptor expression and function in the human T cell compartment has not been reported. Here, we used a P2X7-specific nanobody to assess cell membrane expression and function of P2X7 on peripheral T lymphocyte subsets. The results show that innate-like T cells, which effectively react to innate stimuli by secreting high amounts of pro-inflammatory cytokines, have the highest expression of P2X7 in the human T cell compartment. Using Tγδ cells as example for an innate-like lymphocyte population, we demonstrate that these cells are more sensitive to P2X7 receptor activation than conventional T cells, affecting fundamental cellular mechanisms like calcium signaling and ATP-induced cell death. The increased susceptibility of innate-like T cells to P2X7-mediated cell death provides a mechanism to control their homeostasis under inflammatory conditions. Understanding the expression and function of P2X7 on human immune cells is essential to assume the benefits and consequences of newly developed P2X7-based therapeutic approaches.
Identifiants
pubmed: 36178227
doi: 10.1002/eji.202249932
doi:
Substances chimiques
Receptors, Purinergic P2X7
0
Adenosine Triphosphate
8L70Q75FXE
Cytokines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1805-1818Informations de copyright
© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.
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