Acetylation of Nup62 by TIP60 ensures accurate chromosome segregation in mitosis.

Humans Acetylation Chromosome Segregation Mitosis Nuclear Envelope / metabolism Nuclear Pore Complex Proteins / genetics Lysine Acetyltransferase 5 / metabolism
Nup62 TIP60 acetylation mitosis spindle

Journal

Journal of molecular cell biology
ISSN: 1759-4685
Titre abrégé: J Mol Cell Biol
Pays: United States
ID NLM: 101503669

Informations de publication

Date de publication:
26 12 2022
Historique:
received: 27 03 2022
revised: 14 06 2022
accepted: 29 09 2022
pubmed: 4 10 2022
medline: 16 2 2023
entrez: 3 10 2022
Statut: ppublish

Résumé

Stable transmission of genetic information during cell division requires faithful mitotic spindle assembly and chromosome segregation. In eukaryotic cells, nuclear envelope breakdown (NEBD) is required for proper chromosome segregation. Although a list of mitotic kinases has been implicated in NEBD, how they coordinate their activity to dissolve the nuclear envelope and protein machinery such as nuclear pore complexes was unclear. Here, we identified a regulatory mechanism in which Nup62 is acetylated by TIP60 in human cell division. Nup62 is a novel substrate of TIP60, and the acetylation of Lys432 by TIP60 dissolves nucleoporin Nup62-Nup58-Nup54 complex during entry into mitosis. Importantly, this acetylation-elicited remodeling of nucleoporin complex promotes the distribution of Nup62 to the mitotic spindle, which is indispensable for orchestrating correct spindle orientation. Moreover, suppression of Nup62 perturbs accurate chromosome segregation during mitosis. These results establish a previously uncharacterized regulatory mechanism in which TIP60-elicited nucleoporin dynamics promotes chromosome segregation in mitosis.

Identifiants

DOI: 10.1093/jmcb/mjac056 PMID: 36190325 PMC: PMC9926331
pubmed: 36190325
pii: 6747133
doi: 10.1093/jmcb/mjac056
pmc: PMC9926331
pii:
doi:

Substances chimiques

Nuclear Pore Complex Proteins 0
nuclear pore protein p62 0
KAT5 protein, human EC 2.3.1.48
Lysine Acetyltransferase 5 EC 2.3.1.48

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© The Author(s) (2022). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, CEMCS, CAS.

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Auteurs

Hameed Akbar (H)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Jun Cao (J)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Dongmei Wang (D)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Xiao Yuan (X)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Manjuan Zhang (M)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Saravanakumar Muthusamy (S)

Morehouse School of Medicine, Atlanta, GA 30310, USA.

Xiaoyu Song (X)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
Morehouse School of Medicine, Atlanta, GA 30310, USA.

Xu Liu (X)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Felix Aikhionbare (F)

Morehouse School of Medicine, Atlanta, GA 30310, USA.

Xuebiao Yao (X)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Xinjiao Gao (X)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.

Xing Liu (X)

MOE Key Laboratory for Cellular Dynamics and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China.
CAS Center for Excellence in Molecular and Cell Sciences, Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei 230027, China.
Morehouse School of Medicine, Atlanta, GA 30310, USA.
ORCID: 0000-0002-7480-5085

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Acetylation of Nup62 by TIP60 ensures accurate...
questionsmedicales.fr › Métabolisme › Acylation › Acétylation › Acetylation of Nup62 by TIP60 ensures accurate...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Ségrégation des chromosomes › Acetylation of Nup62 by TIP60 ensures accurate...
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Mitose › Acetylation of Nup62 by TIP60 ensures accurate...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Membranes intracellulaires › Enveloppe nucléaire › Acetylation of Nup62 by TIP60 ensures accurate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Transporteurs nucléocytoplasmiques › Complexe protéique du pore nucléaire › Acetylation of Nup62 by TIP60 ensures accurate...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Acetyltransferases › Lysine acetyltransferases › Histone acetyltransferases › Lysine acetyltransferase 5 › Acetylation of Nup62 by TIP60 ensures accurate...