Exosome loaded hydroxyapatite (HA) scaffold promotes bone regeneration in calvarial defect: an in vivo study.
Bone tissue engineering
Endometrial mesenchymal stem cells
Exosome
Hydroxyl apatite scaffold
Journal
Cell and tissue banking
ISSN: 1573-6814
Titre abrégé: Cell Tissue Bank
Pays: Netherlands
ID NLM: 100965121
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
10
04
2022
accepted:
13
09
2022
medline:
26
5
2023
pubmed:
4
10
2022
entrez:
3
10
2022
Statut:
ppublish
Résumé
In this study, hydroxyapatite (HA) scaffolds were synthesized and characterized, following the osteogenic and angiogenic effects of HA scaffolds with or without endometrial mesenchymal stem stromal cells (hEnSCs) derived Exosomes were investigated in rat animal model with calvaria defect. The X-ray diffraction (XRD) analysis of HA powder formation was confirmed with Joint Corporation of Powder Diffraction Standards (JCPDS) files numbers of 34-0010 and 24-0033A and Ball mill, and sintering manufactured Nano-size particles. Obtained results containing FE-SEM images presented that the surface of scaffolds has a rough and porous structure, which makes them ideal and appropriate for tissue engineering. Additionally, the XRD showed that these scaffolds exhibited a crystallized structure without undergoing phase transformation; meanwhile, manufactured scaffolds consistently release exosomes; moreover, in vivo findings containing hematoxylin-eosin staining, immunohistochemistry, Masson's trichrome staining, and histomorphometric analysis confirmed that our implant has an osteogenic and angiogenic characteristic. So prepared scaffolds containing exosomes can be proposed as a promising substitute in tissue engineering.
Identifiants
pubmed: 36190669
doi: 10.1007/s10561-022-10042-4
pii: 10.1007/s10561-022-10042-4
doi:
Substances chimiques
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
389-400Subventions
Organisme : Semnan University of medical sciences
ID : IR.SEMUMS.REC.1399.048
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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