Synthesis of Unsymmetrical Squaramides as Allosteric GSK-3β Inhibitors Promoting β-Catenin-Mediated Transcription of TCF/LEF in Retinal Pigment Epithelial Cells.


Journal

ChemMedChem
ISSN: 1860-7187
Titre abrégé: ChemMedChem
Pays: Germany
ID NLM: 101259013

Informations de publication

Date de publication:
16 12 2022
Historique:
revised: 03 10 2022
received: 19 08 2022
pubmed: 5 10 2022
medline: 21 12 2022
entrez: 4 10 2022
Statut: ppublish

Résumé

The glycogen synthase kinase 3β (GSK-3β) is a ubiquitous enzyme that is a validated target for the development of potential therapeutics useful in several diseases including retinal degeneration. Aiming at developing an innovative class of allosteric inhibitors of GSK-3β potentially useful for retinal degeneration, we explored the class of squaramides. The developed compounds (6 a-l) were obtained through a nontoxic one-pot synthetic protocol, which employs low-cost goods and avoids any purification step. Ethanol was used as the reaction solvent, simultaneously allowing the pure reaction products' recovery (by precipitation). Out of this set of squaramides, 6 j stood out, from computational and enzymatic converging data, as an ATP non-competitive inhibitor of GSK-3β of micromolar potency. When engaged in cellular studies using retinal pigment epithelial cells (ARPE-19) transfected with a luciferase reporter gene under the control of T-cell factor/lymphoid enhancer factor (TCF/LEF) binding sites, 6 j was able to dose-dependently induce β-catenin nuclear accumulation, as shown by the increased luciferase activity at a concentration of 2.5 μM.

Identifiants

pubmed: 36194001
doi: 10.1002/cmdc.202200456
doi:

Substances chimiques

beta Catenin 0
Glycogen Synthase Kinase 3 beta EC 2.7.11.1
Luciferases EC 1.13.12.-
squaramide 0
TCF Transcription Factors 0
Quinine A7V27PHC7A

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202200456

Informations de copyright

© 2022 Wiley-VCH GmbH.

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Auteurs

Gabriele Carullo (G)

Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Laura Bottoni (L)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Silvia Pasquini (S)

Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Via Fossato di Mortara 17-19, 44121, Ferrara, Italy.

Alessandro Papa (A)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Chiara Contri (C)

Department of Translational Medicine, University of Ferrara, Via Fossato di Mortara 17-19, 44121, Ferrara, Italy.

Simone Brogi (S)

Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126, Pisa, Italy.

Vincenzo Calderone (V)

Department of Pharmacy, University of Pisa, Via Bonanno 6, 56126, Pisa, Italy.

Maurizio Orlandini (M)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Sandra Gemma (S)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Katia Varani (K)

Department of Translational Medicine, University of Ferrara, Via Fossato di Mortara 17-19, 44121, Ferrara, Italy.

Stefania Butini (S)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Federico Galvagni (F)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Fabrizio Vincenzi (F)

Department of Translational Medicine, University of Ferrara, Via Fossato di Mortara 17-19, 44121, Ferrara, Italy.

Giuseppe Campiani (G)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

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