Phosphorylated histone variant γH2Av is associated with chromatin insulators in Drosophila.
Animals
Chromatin
/ genetics
DNA
/ metabolism
Drosophila
/ genetics
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Histones
/ genetics
Insulator Elements
/ genetics
Microtubule-Associated Proteins
/ genetics
Nuclear Proteins
/ genetics
Phosphoric Monoester Hydrolases
/ genetics
Polytene Chromosomes
/ genetics
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
30
03
2022
accepted:
24
08
2022
revised:
17
10
2022
pubmed:
6
10
2022
medline:
20
10
2022
entrez:
5
10
2022
Statut:
epublish
Résumé
Chromatin insulators are responsible for orchestrating long-range interactions between enhancers and promoters throughout the genome and align with the boundaries of Topologically Associating Domains (TADs). Here, we demonstrate an association between gypsy insulator proteins and the phosphorylated histone variant H2Av (γH2Av), normally a marker of DNA double strand breaks. Gypsy insulator components colocalize with γH2Av throughout the genome, in polytene chromosomes and in diploid cells in which Chromatin IP data shows it is enriched at TAD boundaries. Mutation of insulator components su(Hw) and Cp190 results in a significant reduction in γH2Av levels in chromatin and phosphatase inhibition strengthens the association between insulator components and γH2Av and rescues γH2Av localization in insulator mutants. We also show that γH2Av, but not H2Av, is a component of insulator bodies, which are protein condensates that form during osmotic stress. Phosphatase activity is required for insulator body dissolution after stress recovery. Together, our results implicate the H2A variant with a novel mechanism of insulator function and boundary formation.
Identifiants
pubmed: 36197938
doi: 10.1371/journal.pgen.1010396
pii: PGENETICS-D-22-00400
pmc: PMC9576066
doi:
Substances chimiques
CP190 protein, Drosophila
0
Chromatin
0
Drosophila Proteins
0
Histones
0
Microtubule-Associated Proteins
0
Nuclear Proteins
0
DNA
9007-49-2
Phosphoric Monoester Hydrolases
EC 3.1.3.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010396Subventions
Organisme : NIH HHS
ID : P40 OD010949
Pays : United States
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH108956
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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