Combination of Sterile Injury and Microbial Contamination to Model Post-surgical Peritoneal Adhesions in Mice.
Cecal ligation and puncture
Mice
Microbe contamination
Peritoneal adhesion index
Peritoneal button
Peritoneum
Post-surgical adhesion
Surgery
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Aug 2022
20 Aug 2022
Historique:
received:
01
04
2022
revised:
26
06
2022
accepted:
12
07
2022
entrez:
6
10
2022
pubmed:
7
10
2022
medline:
7
10
2022
Statut:
epublish
Résumé
Abdominal surgeries are frequently associated with the development of post-surgical adhesions. These are irreversible fibrotic scar bands that appear between abdominal organs and the abdominal wall. Patients suffering from adhesions are at risk of severe complications, such as small bowel obstruction, chronic pelvic pain, or infertility. To date, no cure exists, and the understanding of underlying molecular mechanisms of adhesion formation is incomplete. The current paradigm largely relies on sterile injury mouse models. However, abdominal surgeries in human patients are rarely completely sterile procedures. Here, we describe a modular surgical procedure for simultaneous or separate induction of sterile injury and microbial contamination. Combined, these insults synergistically lead to adhesion formation in the mouse peritoneal cavity. Surgical trauma is confined to a localized sterile injury of the peritoneum. Microbial contamination of the peritoneal cavity is induced by a limited perforation of the microbe-rich large intestine or by injection of fecal content. The presented protocol extends previous injury-based adhesion models by an additional insult through microbial contamination, which may more adequately model the clinical context of abdominal surgery. Graphical abstract.
Identifiants
pubmed: 36199704
doi: 10.21769/BioProtoc.4491
pii: e4491
pmc: PMC9486694
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2022 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing interests.
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