Development of a novel bioengineered 3D brain-like tissue for studying primary blast-induced traumatic brain injury.
RRID:AB_1074620
RRID:AB_225675
RRID:AB_2633275
RRID:AB_2633281
RRID:AB_2762845
RRID:AB_2921338
RRID:AB_2921339
RRID:CVCL_9115
RRID:CVCL_II76
RRID:SCR_001622
RRID:SCR_002798
RRID:SCR_003070
RRID:SCR_008426
RRID:SCR_017377
RRID:SCR_018163
RRID:SCR_019732
biomarkers
blast
brain injuries
cell culture techniques
coculture techniques
traumatic
Journal
Journal of neuroscience research
ISSN: 1097-4547
Titre abrégé: J Neurosci Res
Pays: United States
ID NLM: 7600111
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
revised:
04
08
2022
received:
28
04
2022
accepted:
29
08
2022
pubmed:
7
10
2022
medline:
25
11
2022
entrez:
6
10
2022
Statut:
ppublish
Résumé
Primary blast injury is caused by the direct impact of an overpressurization wave on the body. Due to limitations of current models, we have developed a novel approach to study primary blast-induced traumatic brain injury. Specifically, we employ a bioengineered 3D brain-like human tissue culture system composed of collagen-infused silk protein donut-like hydrogels embedded with human IPSC-derived neurons, human astrocytes, and a human microglial cell line. We have utilized this system within an advanced blast simulator (ABS) to expose the 3D brain cultures to a blast wave that can be precisely controlled. These 3D cultures are enclosed in a 3D-printed surrogate skull-like material containing media which are then placed in a holder apparatus inside the ABS. This allows for exposure to the blast wave alone without any secondary injury occurring. We show that blast induces an increase in lactate dehydrogenase activity and glutamate release from the cultures, indicating cellular injury. Additionally, we observe a significant increase in axonal varicosities after blast. These varicosities can be stained with antibodies recognizing amyloid precursor protein. The presence of amyloid precursor protein deposits may indicate a blast-induced axonal transport deficit. After blast injury, we find a transient release of the known TBI biomarkers, UCHL1 and NF-H at 6 h and a delayed increase in S100B at 24 and 48 h. This in vitro model will enable us to gain a better understanding of clinically relevant pathological changes that occur following primary blast and can also be utilized for discovery and characterization of biomarkers.
Substances chimiques
Amyloid beta-Protein Precursor
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3-19Informations de copyright
© 2022 Wiley Periodicals LLC. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
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