Distinct role of mitochondrial function and protein kinase C in intimal and medial calcification
drug repurposing
matrix mineralization
mitochondrial function
protein kinase C
vascular calcification
vascular smooth muscle cells
Journal
Frontiers in cardiovascular medicine
ISSN: 2297-055X
Titre abrégé: Front Cardiovasc Med
Pays: Switzerland
ID NLM: 101653388
Informations de publication
Date de publication:
2022
2022
Historique:
received:
01
06
2022
accepted:
15
08
2022
entrez:
7
10
2022
pubmed:
8
10
2022
medline:
8
10
2022
Statut:
epublish
Résumé
Vascular calcification (VC) is a major risk factor for cardiovascular morbidity and mortality. Depending on the location of mineral deposition within the arterial wall, VC is classified as intimal and medial calcification. Using Human coronary artery SMCs were cultured in osteogenic medium (OM) or high calcium phosphate medium (CaP) to induce a mineralized extracellular matrix. OM induces osteoblast-like differentiation of SMCs-a key process in intimal calcification during atherosclerotic plaque remodeling. CaP mimics hyperphosphatemia, associated with chronic kidney disease-a risk factor for medial calcification. Transcriptomic analysis revealed distinct gene expression profiles of OM and CaP-calcifying SMCs. OM and CaP-treated SMCs shared 107 differentially regulated genes related to SMC contraction and metabolism. Real-time extracellular efflux analysis demonstrated decreased mitochondrial respiration and glycolysis in CaP-treated SMCs compared to increased mitochondrial respiration without altered glycolysis in OM-treated SMCs. Subsequent kinome and Our direct comparison results of two
Identifiants
pubmed: 36204585
doi: 10.3389/fcvm.2022.959457
pmc: PMC9530266
doi:
Types de publication
Journal Article
Langues
eng
Pagination
959457Informations de copyright
Copyright © 2022 Heuschkel, Babler, Heyn, van der Vorst, Steenman, Gesper, Kappel, Magne, Gouëffic, Kramann, Jahnen-Dechent, Marx, Quillard and Goettsch.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer JE declared a shared affiliation with one of the author RK to the handling editor at the time of review.
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