Immunocytochemistry of Acutely Isolated Adrenal Medullary Chromaffin Cells.
Acutely isolated cell
Adrenal medullary chromaffin cell
Confocal microscopy
Immunocytochemistry
Immunofluorescence
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
7
10
2022
pubmed:
8
10
2022
medline:
12
10
2022
Statut:
ppublish
Résumé
Immunocytochemistry enables the detection and localization of proteins in cells that are acutely dissociated or in culture. There are advantages and disadvantages to the use of cultured cells for immunocytochemistry. One of the advantages is that cultured cells can be used for one or more weeks after the dissociation of cells, whereas one of the disadvantages is that the properties of cells in culture might change under artificial conditions. On the other hand, acutely dissociated cells are expected to have the original properties of cells because almost all procedures before fixation, except for enzymatic digestion, are carried out at low temperatures. Here, we describe how adrenal medullary cells of small animals are acutely dissociated for immunostaining.
Identifiants
pubmed: 36205885
doi: 10.1007/978-1-0716-2671-9_3
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
35-42Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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