Recording of Chromaffin Cell Electrical Activity In Situ in Acute Adrenal Slices.
Action potential
Acute adrenal slice
Chromaffin cell
Excitability
Mouse
Rat
Stimulus-secretion coupling
Synaptic activity
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
7
10
2022
pubmed:
8
10
2022
medline:
12
10
2022
Statut:
ppublish
Résumé
Because catecholamines secretion mainly relies on the excitable nature of adrenal chromaffin cells, monitoring their electrical activity is an essential step in assessing the adrenal medullary tissue function. The difficult access to the gland in vivo allows only population activity to be recorded in this condition. In vitro preparations allow recordings of spontaneous or evoked activity from single or multiple cells, depending on the biological samples used (dissociated chromaffin cells versus adrenal tissue preparations). In this chapter, I provide a detailed description of the techniques used for electrophysiological recordings in rodent chromaffin cells in acute adrenal slices, using the patch-clamp technique. This methodology allows preservation of the tissue integrity and detection of action potentials, synaptic activity, and secretory events; it is thus suitable for the study of adrenomedullary activity-secretion coupling.
Identifiants
pubmed: 36205891
doi: 10.1007/978-1-0716-2671-9_9
doi:
Substances chimiques
Catecholamines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113-127Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Guerineau NC, Desarmenien MG, Carabelli V, Carbone E (2012) Functional chromaffin cell plasticity in response to stress: focus on nicotinic, gap junction, and voltage-gated Ca
doi: 10.1007/s12031-012-9707-7
Desarmenien MG, Jourdan C, Toutain B, Vessieres E, Hormuzdi SG, Guerineau NC (2013) Gap junction signalling is a stress-regulated component of adrenal neuroendocrine stimulus-secretion coupling in vivo. Nat Commun 4:2938
doi: 10.1038/ncomms3938
Guerineau NC (2020) Cholinergic and peptidergic neurotransmission in the adrenal medulla: a dynamic control of stimulus-secretion coupling. IUBMB Life 72:553–567
doi: 10.1002/iub.2117
Colomer C, Lafont C, Guerineau NC (2008) Stress-induced intercellular communication remodeling in the rat adrenal medulla. Ann N Y Acad Sci 1148:106–111
doi: 10.1196/annals.1410.040
Kidokoro Y, Ritchie AK (1980) Chromaffin cell action potentials and their possible role in adrenaline secretion from rat adrenal medulla. J Physiol 307:199–216
doi: 10.1113/jphysiol.1980.sp013431
Zhou Z, Misler S (1995) Action potential-induced quantal secretion of catecholamines from rat adrenal chromaffin cells. J Biol Chem 270:3498–3505
doi: 10.1074/jbc.270.8.3498
Barbara JG, Poncer JC, McKinney RA, Takeda K (1998) An adrenal slice preparation for the study of chromaffin cells and their cholinergic innervation. J Neurosci Methods 80:181–189
doi: 10.1016/S0165-0270(97)00200-8
Albillos A, Neher E, Moser T (2000) R-type Ca
doi: 10.1523/JNEUROSCI.20-22-08323.2000
Hernandez A, Segura-Chama P, Jimenez N, Garcia AG, Hernandez-Guijo JM, Hernandez-Cruz A (2011) Modulation by endogenously released ATP and opioids of chromaffin cell calcium channels in mouse adrenal slices. Am J Physiol Cell Physiol 300:C610–C623
doi: 10.1152/ajpcell.00380.2010
Hill J, Chan SA, Kuri B, Smith C (2011) Pituitary adenylate cyclase-activating peptide (PACAP) recruits low voltage-activated T-type calcium influx under acute sympathetic stimulation in mouse adrenal chromaffin cells. J Biol Chem 286:42459–42469
doi: 10.1074/jbc.M111.289389
Milman A, Venteo S, Bossu JL, Fontanaud P, Monteil A, Lory P, Guerineau NC (2021) A sodium background conductance controls the spiking pattern of mouse adrenal chromaffin cells in situ. J Physiol 599:1855–1883
doi: 10.1113/JP281044
Chan SA, Polo-Parada L, Smith C (2005) Action potential stimulation reveals an increased role for P/Q-calcium channel-dependent exocytosis in mouse adrenal tissue slices. Arch Biochem Biophys 435:65–73
doi: 10.1016/j.abb.2004.12.005
Rae J, Cooper K, Gates P, Watsky M (1991) Low access resistance perforated patch recordings using amphotericin B. J Neurosci Methods 37:15–26
doi: 10.1016/0165-0270(91)90017-T
De Nardi F, Lefort C, Breard D, Richomme P, Legros C, Guerineau NC (2017) Monitoring the secretory behavior of the rat adrenal medulla by high-performance liquid chromatography-based catecholamine assay from slice supernatants. Front Endocrinol (Lausanne) 8:248
doi: 10.3389/fendo.2017.00248
Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85–100
doi: 10.1007/BF00656997
Martin AO, Mathieu MN, Chevillard C, Guerineau NC (2001) Gap junctions mediate electrical signaling and ensuing cytosolic Ca
doi: 10.1523/JNEUROSCI.21-15-05397.2001
Martin AO, Mathieu MN, Guerineau NC (2003) Evidence for long-lasting cholinergic control of gap junctional communication between adrenal chromaffin cells. J Neurosci 23:3669–3678
doi: 10.1523/JNEUROSCI.23-09-03669.2003
Martin AO, Alonso G, Guerineau NC (2005) Agrin mediates a rapid switch from electrical coupling to chemical neurotransmission during synaptogenesis. J Cell Biol 169:503–514
doi: 10.1083/jcb.200411054
Colomer C, Olivos Ore LA, Coutry N, Mathieu MN, Arthaud S, Fontanaud P, Iankova I, Macari F, Thouennon E, Yon L, Anouar Y, Guerineau NC (2008) Functional remodeling of gap junction-mediated electrical communication between adrenal chromaffin cells in stressed rats. J Neurosci 28:6616–6626
doi: 10.1523/JNEUROSCI.5597-07.2008
Guerineau NC, Monteil A, Lory P (2021) Sodium background currents in endocrine/neuroendocrine cells: towards unraveling channel identity and contribution in hormone secretion. Front Neuroendocrinol 63:100947
doi: 10.1016/j.yfrne.2021.100947
Kuri BA, Chan SA, Smith CB (2009) PACAP regulates immediate catecholamine release from adrenal chromaffin cells in an activity-dependent manner through a protein kinase C-dependent pathway. J Neurochem 110:1214–1225
doi: 10.1111/j.1471-4159.2009.06206.x
Albinana E, Segura-Chama P, Baraibar AM, Hernandez-Cruz A, Hernandez-Guijo JM (2015) Different contributions of calcium channel subtypes to electrical excitability of chromaffin cells in rat adrenal slices. J Neurochem 133:511–521
doi: 10.1111/jnc.13055
Neyton J, Trautmann A (1985) Single-channel currents of an intercellular junction. Nature 317:331–335
doi: 10.1038/317331a0
Hill J, Lee SK, Samasilp P, Smith C (2012) Pituitary adenylate cyclase-activating peptide enhances electrical coupling in the mouse adrenal medulla. Am J Physiol Cell Physiol 303:C257–C266
doi: 10.1152/ajpcell.00119.2012
Perkins KL (2006) Cell-attached voltage-clamp and current-clamp recording and stimulation techniques in brain slices. J Neurosci Methods 154:1–18
doi: 10.1016/j.jneumeth.2006.02.010
Albillos A, Dernick G, Horstmann H, Almers W, Alvarez de Toledo G, Lindau M (1997) The exocytotic event in chromaffin cells revealed by patch amperometry. Nature 389:509–512
doi: 10.1038/39081
Dernick G, Alvarez de Toledo G, Lindau M (2003) Exocytosis of single chromaffin granules in cell-free inside-out membrane patches. Nat Cell Biol 5:358–362
doi: 10.1038/ncb956
Dernick G, Gong LW, Tabares L, Alvarez de Toledo G, Lindau M (2005) Patch amperometry: high-resolution measurements of single-vesicle fusion and release. Nat Methods 2:699–708
doi: 10.1038/nmeth0905-699