Immune Profiling and Multiplexed Label-Free Detection of 2D MXenes by Mass Cytometry and High-Dimensional Imaging.
MXenes
biocompatibility
biomedical applications
immune system
nanomedicine
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
22
07
2022
received:
07
06
2022
pubmed:
8
10
2022
medline:
15
11
2022
entrez:
7
10
2022
Statut:
ppublish
Résumé
There is a critical unmet need to detect and image 2D materials within single cells and tissues while surveying a high degree of information from single cells. Here, a versatile multiplexed label-free single-cell detection strategy is proposed based on single-cell mass cytometry by time-of-flight (CyTOF) and ion-beam imaging by time-of-flight (MIBI-TOF). This strategy, "Label-free sINgle-cell tracKing of 2D matErials by mass cytometry and MIBI-TOF Design" (LINKED), enables nanomaterial detection and simultaneous measurement of multiple cell and tissue features. As a proof of concept, a set of 2D materials, transition metal carbides, nitrides, and carbonitrides (MXenes), is selected to ensure mass detection within the cytometry range while avoiding overlap with more than 70 currently available tags, each able to survey multiple biological parameters. First, their detection and quantification in 15 primary human immune cell subpopulations are demonstrated. Together with the detection, mass cytometry is used to capture several biological aspects of MXenes, such as their biocompatibility and cytokine production after their uptake. Through enzymatic labeling, MXenes' mediation of cell-cell interactions is simultaneously evaluated. In vivo biodistribution experiments using a mixture of MXenes in mice confirm the versatility of the detection strategy and reveal MXene accumulation in the liver, blood, spleen, lungs, and relative immune cell subtypes. Finally, MIBI-TOF is applied to detect MXenes in different organs revealing their spatial distribution. The label-free detection of 2D materials by mass cytometry at the single-cell level, on multiple cell subpopulations and in multiple organs simultaneously, will enable exciting new opportunities in biomedicine.
Identifiants
pubmed: 36207284
doi: 10.1002/adma.202205154
doi:
Substances chimiques
Transition Elements
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2205154Subventions
Organisme : Israel Science Foundation
ID : 3830/21
Organisme : Center for New Scientists
Organisme : Weizmann Institute of Science
Organisme : NHLBI NIH HHS
ID : R35 HL145241
Pays : United States
Organisme : Enoch Foundation Research Fund
Organisme : European Research Council
ID : 94811
Pays : International
Organisme : Israeli Council for Higher Education
Organisme : Sidra Medicine Internal Funds
ID : SDR400025
Organisme : Israel Science Foundation
ID : 2481/20
Organisme : European Union's Horizon 2020 Research and Innovation Programme
ID : 734381
Organisme : Weizmann Data Science Research Center
Organisme : Israel Precision Medicine Partnership
Organisme : European Union's Horizon 2020 Research and Innovation Programme
ID : 101029140
Organisme : Schwartz/Reisman Collaborative Science Program
Organisme : National Science Foundation
ID : DMR-2035007
Informations de copyright
© 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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