Marker-free co-selection for successive rounds of prime editing in human cells.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 10 2022
07 10 2022
Historique:
received:
03
12
2021
accepted:
27
09
2022
entrez:
7
10
2022
pubmed:
8
10
2022
medline:
12
10
2022
Statut:
epublish
Résumé
Prime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. However, efficiency remains a key challenge in a broad range of human cell types. In this work, we design a robust co-selection strategy through coediting of the ubiquitous and essential sodium/potassium pump (Na
Identifiants
pubmed: 36207338
doi: 10.1038/s41467-022-33669-z
pii: 10.1038/s41467-022-33669-z
pmc: PMC9546848
doi:
Substances chimiques
DNA
9007-49-2
Sodium
9NEZ333N27
Sodium-Potassium-Exchanging ATPase
EC 7.2.2.13
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5909Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2022. The Author(s).
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