Engineering triacylglycerol accumulation in duckweed (Lemna japonica).
DGAT
Lemna minor
WRI1
duckweed
plant oil
triacylglycerol
Journal
Plant biotechnology journal
ISSN: 1467-7652
Titre abrégé: Plant Biotechnol J
Pays: England
ID NLM: 101201889
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
revised:
08
09
2022
received:
16
06
2022
accepted:
30
09
2022
pubmed:
10
10
2022
medline:
1
2
2023
entrez:
9
10
2022
Statut:
ppublish
Résumé
Duckweeds are amongst the fastest growing of higher plants, making them attractive high-biomass targets for biofuel feedstock production. Their fronds have high rates of fatty acid synthesis to meet the demand for new membranes, but triacylglycerols (TAG) only accumulate to very low levels. Here we report on the engineering of Lemna japonica for the synthesis and accumulation of TAG in its fronds. This was achieved by expression of an estradiol-inducible cyan fluorescent protein-Arabidopsis WRINKLED1 fusion protein (CFP-AtWRI1), strong constitutive expression of a mouse diacylglycerol:acyl-CoA acyltransferase2 (MmDGAT), and a sesame oleosin variant (SiOLE(*)). Individual expression of each gene increased TAG accumulation by 1- to 7-fold relative to controls, while expression of pairs of these genes increased TAG by 7- to 45-fold. In uninduced transgenics containing all three genes, TAG accumulation increased by 45-fold to 3.6% of dry weight (DW) without severely impacting growth, and by 108-fold to 8.7% of DW after incubation on medium containing 100 μm estradiol for 4 days. TAG accumulation was accompanied by an increase in total fatty acids of up to three-fold to approximately 15% of DW. Lipid droplets from fronds of all transgenic lines were visible by confocal microscopy of BODIPY-stained fronds. At a conservative 12 tonnes (dry matter) per acre and 10% (DW) TAG, duckweed could produce 350 gallons of oil/acre/year, approximately seven-fold the yield of soybean, and similar to that of oil palm. These findings provide the foundation for optimizing TAG accumulation in duckweed and present a new opportunity for producing biofuels and lipidic bioproducts.
Identifiants
pubmed: 36209479
doi: 10.1111/pbi.13943
pmc: PMC9884027
doi:
Substances chimiques
Triglycerides
0
Lipids
0
Fatty Acids
0
WRINKLED1 protein, Arabidopsis
0
Transcription Factors
0
Arabidopsis Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
317-330Subventions
Organisme : NCI NIH HHS
ID : P30 CA045508
Pays : United States
Informations de copyright
© 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Références
Nat Chem Biol. 2016 Feb;12(2):82-6
pubmed: 26641934
FEBS Lett. 2013 Feb 14;587(4):364-9
pubmed: 23313251
Nat Commun. 2014;5:3311
pubmed: 24548928
PLoS One. 2019 Nov 25;14(11):e0224137
pubmed: 31765385
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13018-23
pubmed: 9789033
Plant Biol (Stuttg). 2015 Jan;17 Suppl 1:59-65
pubmed: 24989135
Plant Cell. 2018 Oct;30(10):2616-2627
pubmed: 30249634
RNA Biol. 2018;15(6):756-762
pubmed: 29578372
J Lipid Res. 2019 Jun;60(6):1112-1120
pubmed: 30936184
Sci Total Environ. 2018 Sep 1;634:1034-1041
pubmed: 29660861
J Agric Food Chem. 1980 Jul-Aug;28(4):848-50
pubmed: 7462500
Plant Cell. 2021 Oct 11;33(10):3207-3234
pubmed: 34273173
Nucleic Acids Res. 1987 Oct 26;15(20):8125-48
pubmed: 3313277
Plant Physiol Biochem. 2011 Jan;49(1):77-81
pubmed: 21041098
Mol Plant. 2013 Nov;6(6):1814-29
pubmed: 23713076
Genome Res. 2020 Dec 23;:
pubmed: 33361111
Plant Physiol. 2019 Sep;181(1):55-62
pubmed: 31209126
Plant Biol (Stuttg). 2015 Sep;17(5):1066-72
pubmed: 25950142
BMC Plant Biol. 2013 Dec 05;13:201
pubmed: 24308551
Plant Physiol. 2014 Jan;164(1):455-65
pubmed: 24204024
PLoS One. 2011 Feb 18;6(2):e16765
pubmed: 21364738
Biophys Rev. 2022 Jan 8;14(1):257-266
pubmed: 35340610
Metab Eng. 2017 Jan;39:237-246
pubmed: 27993560
BMC Plant Biol. 2015 Aug 08;15:192
pubmed: 26253704
J Biol Chem. 2004 Mar 19;279(12):11767-76
pubmed: 14668353
Plant Direct. 2021 Sep 06;5(9):e343
pubmed: 34514289
Planta. 2019 Jul;250(1):79-94
pubmed: 30919065
Plant Biotechnol J. 2017 Jul;15(7):824-836
pubmed: 27987528
Plant Physiol. 1998 Sep;118(1):91-101
pubmed: 9733529
Nat Genet. 2000 May;25(1):87-90
pubmed: 10802663
Plant Biotechnol J. 2019 Nov;17(11):2143-2152
pubmed: 30972865
Plant J. 2003 Mar;33(5):949-56
pubmed: 12609035
Plant Biotechnol J. 2014 Feb;12(2):231-9
pubmed: 24151938
Plant Physiol. 2015 Nov;169(3):1836-47
pubmed: 26419778
Plant Physiol. 2013 Jun;162(2):626-39
pubmed: 23616604
Plant Biotechnol J. 2009 Dec;7(9):914-24
pubmed: 19843252
Bioeng Bugs. 2012 Jan 1;3(1):38-43
pubmed: 22126803
Plant Biotechnol J. 2020 Feb 18;:
pubmed: 32069385
Plant Biotechnol J. 2019 Jan;17(1):220-232
pubmed: 29873878
Front Plant Sci. 2021 Mar 08;12:625670
pubmed: 33763089
Prog Lipid Res. 2010 Apr;49(2):108-19
pubmed: 19857520
ACS Synth Biol. 2014 Nov 21;3(11):839-43
pubmed: 24933124
Plant Cell. 2017 Apr;29(4):871-889
pubmed: 28314829
Plant J. 2000 Oct;24(2):265-73
pubmed: 11069700
Protein J. 2020 Aug;39(4):366-376
pubmed: 32472380
J Exp Bot. 2020 Apr 6;71(7):2351-2361
pubmed: 31679036
Plant Physiol. 2013 Jul;162(3):1282-9
pubmed: 23686420
Plant Cell Rep. 2007 Sep;26(9):1511-9
pubmed: 17492286
Front Plant Sci. 2020 Mar 06;11:215
pubmed: 32210994
Biotechnol Biofuels. 2015 Nov 25;8:188
pubmed: 26609323
Plants (Basel). 2021 Jul 30;10(8):
pubmed: 34451621
Science. 1992 Oct 9;258(5080):287-92
pubmed: 17835129
J Cell Biol. 1989 Feb;108(2):229-41
pubmed: 2645293
J Lipid Res. 2012 Feb;53(2):215-26
pubmed: 22045929
Plant Biotechnol J. 2016 Feb;14(2):661-9
pubmed: 26058948
Sci Rep. 2019 Mar 1;9(1):3234
pubmed: 30824726
J Plant Physiol. 2007 Dec;164(12):1656-64
pubmed: 17296247
Plant Physiol. 2002 Apr;128(4):1200-11
pubmed: 11950969
Plant Methods. 2018 Aug 25;14:71
pubmed: 30159002
BMC Plant Biol. 2010 Sep 16;10:205
pubmed: 20846439
Front Chem. 2018 Oct 15;6:479
pubmed: 30374437