Microbial characteristics of dental caries in HIV positive individuals.
DMFT index
dental caries
human immunodeficiency virus
microbial co-occurrence networks
oral microbiota
Journal
Frontiers in oral health
ISSN: 2673-4842
Titre abrégé: Front Oral Health
Pays: Switzerland
ID NLM: 9918227262706676
Informations de publication
Date de publication:
2022
2022
Historique:
received:
27
07
2022
accepted:
31
08
2022
entrez:
10
10
2022
pubmed:
11
10
2022
medline:
11
10
2022
Statut:
epublish
Résumé
Dental caries is a multifactorial disease that affects many people. Even though microorganisms play a crucial role in causing dental caries, diagnosis is routinely macroscopic. In order to improve early detection especially in HIV patients who are disproportionately affected, there is need to reconcile the macroscopic and microscopic characteristics of dental caries. Therefore, the aim of this study was to characterize the oral microbiota profile along the decayed, missing, filled teeth (DMFT) index using amplicon sequencing data. Amplicon sequencing of the V6-V8 region of the We characterized the oral microbiota into 2,093 operational taxonomic units (OTUs), 21 phyla and 239 genera from 2.6 million high quality sequence reads. While oral microbiota did not cluster participants into distinct groups that track with the DMFT index, we observed the following: (a) The proportion of accessory microbiota was highest in the high DMFT category while the core size (∼50% of richness) remained relatively stable across all categories. (b) The abundance of core genera such as Although oral microbial shifts along the DMFT index were not distinct, we demonstrated the potential utility of microbiota dynamics to characterize oral disease. Therefore, we propose a microbial framework using the DMFT index to better understand dental caries among HIV positive people in resource limited settings.
Sections du résumé
Background
UNASSIGNED
Dental caries is a multifactorial disease that affects many people. Even though microorganisms play a crucial role in causing dental caries, diagnosis is routinely macroscopic. In order to improve early detection especially in HIV patients who are disproportionately affected, there is need to reconcile the macroscopic and microscopic characteristics of dental caries. Therefore, the aim of this study was to characterize the oral microbiota profile along the decayed, missing, filled teeth (DMFT) index using amplicon sequencing data.
Methods
UNASSIGNED
Amplicon sequencing of the V6-V8 region of the
Results
UNASSIGNED
We characterized the oral microbiota into 2,093 operational taxonomic units (OTUs), 21 phyla and 239 genera from 2.6 million high quality sequence reads. While oral microbiota did not cluster participants into distinct groups that track with the DMFT index, we observed the following: (a) The proportion of accessory microbiota was highest in the high DMFT category while the core size (∼50% of richness) remained relatively stable across all categories. (b) The abundance of core genera such as
Conclusions
UNASSIGNED
Although oral microbial shifts along the DMFT index were not distinct, we demonstrated the potential utility of microbiota dynamics to characterize oral disease. Therefore, we propose a microbial framework using the DMFT index to better understand dental caries among HIV positive people in resource limited settings.
Identifiants
pubmed: 36211252
doi: 10.3389/froh.2022.1004930
pmc: PMC9533146
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1004930Subventions
Organisme : FIC NIH HHS
ID : D43 TW010132
Pays : United States
Informations de copyright
© 2022 Kalanzi, Mayanja-Kizza, Nakanjako, Semitala, Mboowa, Mbabali, Kigozi, Katabazi, Sserwadda, Kateete, Achan, Sewankambo and Muwonge.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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