Construction of chicken infectious anemia virus infectious clone and study on its pathogenicity.
VP3 fusion protein
chicken infectious anemia virus
fowl pox virus
infectious clone
pathogenicity
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2022
2022
Historique:
received:
11
08
2022
accepted:
06
09
2022
entrez:
10
10
2022
pubmed:
11
10
2022
medline:
11
10
2022
Statut:
epublish
Résumé
Chicken infectious anemia virus (CIAV) can be transmitted through contaminated live poultry vaccine. However, the pathogenicity of contaminated CIAV strains is rarely reported. Previously, the chickens showed the typical symptoms of anemia after using the attenuated live fowl pox virus (FPV) vaccine. Therefore, exogenous CIAV contamination was suspected. We detected anti-CIAV antibodies in SPF chicks vaccinated with the FPV vaccine. CIAV contamination was confirmed in the FPV vaccine, and the CIAV strain was named JS2020-FPV. This study aims to rescue JS2020-FPV by reverse genetic assays and investigate its pathogenicity. Firstly, double-copies infectious clone of JS2020-FPV was constructed. For the pathogenicity study, infectious clone of JS2020-FPV was used to inoculate 1-day-old SPF chicks. The typical symptoms of anemia were observed in the JS2020-PFV group 14 days post inoculation. The hematocrit and body weight of chicks in the JS2020-PFV group were significantly lower than those in the mock group. Notably, the thymus development index and antibody levels of NDV were lower in chicks in the JS2020-PFV group than those in the mock group. Different degrees of apoptosis of MSB1 and DF-1 were observed after inoculated with the JS2020-FPV VP3 recombinant fusion protein expressed by
Identifiants
pubmed: 36212822
doi: 10.3389/fmicb.2022.1016784
pmc: PMC9539448
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1016784Informations de copyright
Copyright © 2022 Wang, Li, Zahng, Chen, Fang, Chang, Wang and Zhao.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Exp Ther Med. 2012 Jun;3(6):1033-1038
pubmed: 22970013
Poult Sci. 2014 Sep;93(9):2168-74
pubmed: 25002550
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5843-7
pubmed: 9159162
Vet Microbiol. 2018 May;218:52-59
pubmed: 29685221
PLoS One. 2012;7(8):e43422
pubmed: 22912872
J Histochem Cytochem. 2004 Apr;52(4):469-77
pubmed: 15033998
Transbound Emerg Dis. 2018 Aug;65(4):1103-1106
pubmed: 29479824
Front Cell Infect Microbiol. 2021 Feb 02;10:609071
pubmed: 33604306
Dev Comp Immunol. 2000 Mar-Apr;24(2-3):247-55
pubmed: 10717291
Poult Sci. 2017 May 1;96(5):1094-1099
pubmed: 27794542
Avian Dis. 2006 Jun;50(2):209-15
pubmed: 16863069
Avian Pathol. 2020 Feb;49(1):5-14
pubmed: 31259607
Biologicals. 2014 Nov;42(6):346-50
pubmed: 25439092
Avian Dis. 2008 Jun;52(2):245-52
pubmed: 18646453
J Virol. 1994 Jan;68(1):346-51
pubmed: 8254747
Leukemia. 1995 Oct;9 Suppl 1:S118-20
pubmed: 7475302
Poult Sci. 2017 May 1;96(5):1045-1051
pubmed: 27811322
Poult Sci. 2015 Apr;94(4):643-9
pubmed: 25725074
J Virol. 1991 Jun;65(6):3131-9
pubmed: 1851873
Front Vet Sci. 2022 Jul 06;9:930887
pubmed: 35873689
Biomed Res Int. 2017;2017:6707868
pubmed: 28326326
J Virol. 1992 Dec;66(12):7383-8
pubmed: 1331529