Split-Chloramphenicol Acetyl Transferase Assay to Study Protein-Protein Interactions and Ubiquitylation in
Bacterial two hybrid
Chloramphenicol Acetyl Transferase
Protein-protein interaction
Self-ubiquitylation
Ubiquitin E3-ligase
Ubiquitylation readout
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2022
05 Sep 2022
Historique:
received:
03
05
2022
revised:
08
08
2022
accepted:
20
07
2022
entrez:
10
10
2022
pubmed:
11
10
2022
medline:
11
10
2022
Statut:
epublish
Résumé
Protein-protein interactions and protein modifications play central roles in all living organisms. Of the more than 200 types of post-translational modifications, ubiquitylation is the most abundant, and it profoundly regulates the functionality of the eukaryotic proteome. Various
Identifiants
pubmed: 36213104
doi: 10.21769/BioProtoc.4497
pii: e4497
pmc: PMC9501773
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2022 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests GP has equity in Coltac therapeutics LTD, other authors declare no competing interests. Company and patents: Patents: US10982252, US20200385706, and US20210356467.
Références
Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12141-6
pubmed: 9770453
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10340-4
pubmed: 7937952
Nat Biotechnol. 2002 Jun;20(6):619-22
pubmed: 12042868
Mol Microbiol. 2008 Feb;67(4):719-28
pubmed: 18179417
Mol Microbiol. 1997 Nov;26(3):619-20
pubmed: 9402030
J Mol Biol. 2021 Nov 19;433(23):167276
pubmed: 34599943
Nat Biotechnol. 2003 Mar;21(3):315-8
pubmed: 12577067
Cancer Res. 2007 Oct 1;67(19):9472-81
pubmed: 17909057
Nat Biotechnol. 2003 Aug;21(8):921-6
pubmed: 12872131
Nature. 1989 Jul 20;340(6230):245-6
pubmed: 2547163
Science. 1985 Jun 14;228(4705):1315-7
pubmed: 4001944
Nat Commun. 2016 Oct 04;7:12960
pubmed: 27698474
Gene. 1990 Nov 30;96(1):23-8
pubmed: 2265755
Mol Syst Biol. 2007;3:116
pubmed: 17551511
Plant J. 2004 Nov;40(3):428-38
pubmed: 15469500
CSH Protoc. 2006 Jun 01;2006(1):
pubmed: 22485385
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
EMBO J. 2013 Feb 20;32(4):538-51
pubmed: 23361315
J Biotechnol. 2002 Jan;82(3):267-77
pubmed: 11999694
Science. 1999 Jul 30;285(5428):751-3
pubmed: 10427000
Biochemistry. 2012 Jul 24;51(29):5841-50
pubmed: 22702834
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5187-92
pubmed: 9560251