Evaluation of Mitochondrial Turnover Using Fluorescence Microscopy in
Dissection
Drosophila
Fluorescence microscopy
Insecticide
Mitochondria
Mitochondrial turnover
Oxidative stress
Tissue imaging
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2022
05 Sep 2022
Historique:
received:
15
05
2022
revised:
24
07
2022
accepted:
24
07
2022
entrez:
10
10
2022
pubmed:
11
10
2022
medline:
11
10
2022
Statut:
epublish
Résumé
Mitochondrial dysfunction is associated with perturbations in the cellular oxidative status, changes in energy production and metabolic rate, and the onset of pathological processes. Classic methods of assessing mitochondrial dysfunction rely on indirect measures, such as evaluating mitochondrial DNA copy numbers, or direct but more costly and skilled techniques, such as electron microscopy. The protocol presented here was recently implemented to evaluate mitochondrial dysfunction in response to insecticide exposure in
Identifiants
pubmed: 36213109
doi: 10.21769/BioProtoc.4498
pii: e4498
pmc: PMC9501756
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests The author declares no conflicts of interest.
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