Photoacoustic imaging for the study of oxygen saturation and total hemoglobin in bone healing and non-union formation.
Angiogenesis
Bone healing
Mice
Microvessel density
Non-union
Oxygen saturation
Photoacoustic imaging
Vascularization
Journal
Photoacoustics
ISSN: 2213-5979
Titre abrégé: Photoacoustics
Pays: Germany
ID NLM: 101622604
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
23
01
2022
revised:
14
08
2022
accepted:
25
09
2022
entrez:
10
10
2022
pubmed:
11
10
2022
medline:
11
10
2022
Statut:
epublish
Résumé
Non-union formation represents a major complication in trauma surgery. Adequate vascularization has been recognized as vital for bone healing. However, the role of vascularization in the pathophysiology of non-union formation remains elusive. This is due to difficulties in studying bone microcirculation in vivo. Therefore, we herein studied in a murine osteotomy model whether photoacoustic imaging may be used to analyze vascularization in bone healing and non-union formation. We found that oxygen saturation within the callus tissue is significantly lower in non-unions compared to unions and further declines over time. Moreover, the amount of total hemoglobin (HbT) within the callus tissue was markedly reduced in non-unions. Correlation analyses showed a strong positive correlation between microvessel density and HbT, indicating that photoacoustically determined HbT is a valid parameter to assess vascularization during bone healing. In summary, photoacoustic imaging is a promising approach to study vascular function and tissue oxygenation in bone regeneration.
Identifiants
pubmed: 36213763
doi: 10.1016/j.pacs.2022.100409
pii: S2213-5979(22)00074-X
pmc: PMC9535319
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100409Informations de copyright
© 2022 Published by Elsevier GmbH.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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