Sirt3 Promotes Chondrogenesis, Chondrocyte Mitochondrial Respiration and the Development of High-Fat Diet-Induced Osteoarthritis in Mice.
HIGH-FAT DIET
METABOLISM
OBESITY
OSTEOARTHRITIS
PRIMARY JUVENILE MURINE CHONDROCYTES
Sirt3
Journal
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
ISSN: 1523-4681
Titre abrégé: J Bone Miner Res
Pays: United States
ID NLM: 8610640
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
revised:
09
09
2022
received:
22
12
2021
accepted:
06
10
2022
pubmed:
11
10
2022
medline:
22
12
2022
entrez:
10
10
2022
Statut:
ppublish
Résumé
Understanding how obesity-induced metabolic stress contributes to synovial joint tissue damage is difficult because of the complex role of metabolism in joint development, maintenance, and repair. Chondrocyte mitochondrial dysfunction is implicated in osteoarthritis (OA) pathology, which motivated us to study the mitochondrial deacetylase enzyme sirtuin 3 (Sirt3). We hypothesized that combining high-fat-diet (HFD)-induced obesity and cartilage Sirt3 loss at a young age would impair chondrocyte mitochondrial function, leading to cellular stress and accelerated OA. Instead, we unexpectedly found that depleting cartilage Sirt3 at 5 weeks of age using Sirt3-flox and Acan-Cre
Identifiants
pubmed: 36214465
doi: 10.1002/jbmr.4721
pmc: PMC10091721
doi:
Substances chimiques
Sirt3 protein, mouse
0
Sirtuin 3
EC 3.5.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2531-2547Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM139763
Pays : United States
Organisme : NIGMS NIH HHS
ID : P30 GM114731
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG049058
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20GM139763
Pays : United States
Informations de copyright
© 2022 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
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