Absence of anti-Besnoitia spp. specific antibodies in European wild lagomorphs from the Iberian Peninsula.
Lepus europaeus
Oryctolagus cuniculus
bovine besnoitiosis
intermediate host
serology
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
20
09
2022
received:
08
06
2022
accepted:
05
10
2022
pubmed:
11
10
2022
medline:
7
2
2023
entrez:
10
10
2022
Statut:
ppublish
Résumé
Besnoitia besnoiti is an apicomplexan parasite whose life cycle is not completely understood. It is assumed that this parasite might have an indirect life cycle with a carnivore as a definitive host able to shed oocysts after the ingestion of mature cysts in tissues of an infected intermediate host. Cattle and wild cervids on the Iberian Peninsula can act as intermediate hosts of B. besnoiti, and exposure to the parasite has been demonstrated in equids. In this study, we aimed to assess the presence of members of the genera Besnoitia in wild lagomorphs from the Iberian Peninsula and the potential role of these host species in the life cycle of B. besnoiti, as all the animals were sampled from 19 regions of the Iberian Peninsula where cases of bovine besnoitiosis have been previously detected. Serum samples (Oryctolagus cuniculus: n = 552; Lepus europaeus: n = 122) were first analysed by ELISA and subsequently confirmed by Western blot (WB). Specific antibodies against B. besnoiti were not found in any sampled animal by WB. In addition, lung samples from a subset of wild rabbits (n = 16) were tested by PCR and Besnoitia spp. DNA was not detected. These results suggest that Besnoitia spp. are unlikely to circulate in wild lagomorphs in the Iberian Peninsula. Thus, lagomorphs are not expected to play a key role in the biological cycle of B. besnoiti. Further studies are necessary to assess whether different micromammal species, such as rodents, can serve as natural reservoirs of Besnoitia spp. in other European regions.
Substances chimiques
Antibodies, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3447-e3454Subventions
Organisme : Complutense University of Madrid
ID : CT65/16
Organisme : Spanish Ministry of Economy and Competitiveness
ID : AGL-2016-75202-R
Organisme : Community of Madrid
ID : PLATESA P2018/BAA-4370
Organisme : Spanish Ministry of Science through a Juan de la Cierva postdoctoral fellowship
ID : FJCI-2016-27875
Organisme : Portuguese Foundation for Science and Technology (FCT)
Informations de copyright
© 2022 Wiley-VCH GmbH.
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