Rapid fractionation of mitochondria from mouse liver and heart reveals in vivo metabolite compartmentation.
in vivo
compartmentation
ischemia
metabolites
mitochondria
rapid fractionation
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
revised:
30
09
2022
received:
03
09
2022
accepted:
07
10
2022
pubmed:
12
10
2022
medline:
26
1
2023
entrez:
11
10
2022
Statut:
ppublish
Résumé
The compartmentation and distribution of metabolites between mitochondria and the rest of the cell is a key parameter of cell signalling and pathology. Here, we have developed a rapid fractionation procedure that enables us to take mouse heart and liver from in vivo and within ~ 30 s stabilise the distribution of metabolites between mitochondria and the cytosol by rapid cooling, homogenisation and dilution. This is followed by centrifugation of mitochondria through an oil layer to separate mitochondrial and cytosolic fractions for subsequent metabolic analysis. Using this procedure revealed the in vivo compartmentation of mitochondrial metabolites and will enable the assessment of the distribution of metabolites between the cytosol and mitochondria during a range of situations in vivo.
Identifiants
pubmed: 36217875
doi: 10.1002/1873-3468.14511
pmc: PMC7614208
mid: EMS164657
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
246-261Subventions
Organisme : Wellcome Trust
ID : 220257/Z/20/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 220257
Pays : United Kingdom
Organisme : UK Research and Innovation
ID : MC_UU_12022/7
Organisme : UK Research and Innovation
ID : MC_UU_00028/4
Organisme : Medical Research Council
ID : MC_UU_12022/6
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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