Reconstitution and biochemical studies of extended synaptotagmin-mediated lipid transport.
Calcium
Endoplasmic reticulum
Extended synaptotagmin
Lipid transfer
Liposome reconstitution
Membrane contact sites
Plasma membrane
SMP
Tethering
Tricalbin
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
11
10
2022
pubmed:
12
10
2022
medline:
14
10
2022
Statut:
ppublish
Résumé
Extended synaptotagmins (E-Syts) are a family of lipid transfer proteins (LTPs) located at the endoplasmic reticulum (ER)-plasma membrane (PM) contact sites in eukaryotic cells. They possess a conserved synaptotagmin-like mitochondrial-lipid-binding protein (SMP) domain and two to five C2 domains. While the membrane tethering function of E-Syts has been well studied in diverse species, recent studies revealed that the mammalian E-Syt1 and its yeast homolog tricalbin 3 (Tcb3) could transport lipids between the opposed membrane. Mechanical studies suggested SYT1 transfers lipids fundamentally through the SMP domain, but the lipid transport requires the regulation of C2 domain-mediated membrane tethering. In addition, both E-Syt1 and Tcb3 are Ca
Identifiants
pubmed: 36220276
pii: S0076-6879(22)00241-5
doi: 10.1016/bs.mie.2022.07.003
pii:
doi:
Substances chimiques
Lipids
0
Mitochondrial Proteins
0
Recombinant Proteins
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-62Informations de copyright
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