Fast Mass Microscopy: Mass Spectrometry Imaging of a Gigapixel Image in 34 Minutes.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
25 10 2022
25 10 2022
Historique:
pubmed:
13
10
2022
medline:
27
10
2022
entrez:
12
10
2022
Statut:
ppublish
Résumé
Mass spectrometry imaging (MSI) maps the spatial distributions of chemicals on surfaces. MSI requires improvements in throughput and spatial resolution, and often one is compromised for the other. In microprobe-mode MSI, improvements in spatial resolution increase the imaging time quadratically, thus limiting the use of high spatial resolution MSI for large areas or sample cohorts and time-sensitive measurements. Here, we bypass this quadratic relationship by combining a Timepix3 detector with a continuously sampling secondary ion mass spectrometry mass microscope. By reconstructing the data into large-field mass images, this new method, fast mass microscopy, enables orders of magnitude higher throughput than conventional MSI albeit yet at lower mass resolution. We acquired submicron, gigapixel images of fingerprints and rat tissue at acquisition speeds of 600,000 and 15,500 pixels s
Identifiants
pubmed: 36223179
doi: 10.1021/acs.analchem.2c02870
pmc: PMC9607864
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14652-14658Références
Nat Methods. 2019 Sep;16(9):925-931
pubmed: 31451764
Angew Chem Int Ed Engl. 2020 Sep 28;59(40):17447-17450
pubmed: 32668069
Anal Chem. 2015 Feb 3;87(3):1437-55
pubmed: 25526173
Nat Med. 2014 Apr;20(4):436-42
pubmed: 24584119
Postdoc J. 2016 Mar;4(3):3-13
pubmed: 27570788
J Am Soc Mass Spectrom. 2021 Apr 7;32(4):872-894
pubmed: 33656885
Nat Methods. 2007 Oct;4(10):828-33
pubmed: 17901873
Nat Protoc. 2007;2(5):1185-96
pubmed: 17546014
J Proteomics. 2012 Aug 30;75(16):4999-5013
pubmed: 22842290
Anal Chem. 2004 Sep 15;76(18):5339-44
pubmed: 15362890
J Am Soc Mass Spectrom. 2011 Jun;22(6):1022-31
pubmed: 21953043
Chem Rev. 2010 May 12;110(5):3237-77
pubmed: 20423155
J Am Soc Mass Spectrom. 2019 Feb;30(2):289-298
pubmed: 30456596
Mass Spectrom Rev. 2007 Jul-Aug;26(4):606-43
pubmed: 17471576
Science. 2013 Dec 6;342(6163):1243259
pubmed: 24311695
J Proteomics. 2014 Jul 31;107:71-82
pubmed: 24686089
Nat Methods. 2017 Jan;14(1):90-96
pubmed: 27842060
Analyst. 2017 Jul 24;142(15):2690-2712
pubmed: 28642940
Anal Bioanal Chem. 2010 Aug;397(8):3409-19
pubmed: 20635080
Nat Methods. 2017 Dec;14(12):1175-1183
pubmed: 29131162
J Mass Spectrom. 2005 Feb;40(2):160-8
pubmed: 15706616
Rapid Commun Mass Spectrom. 2015 Dec 15;29(23):2195-203
pubmed: 26522310
Proteomics. 2016 Jun;16(11-12):1678-89
pubmed: 27060368
NPJ Precis Oncol. 2019 Jul 4;3:17
pubmed: 31286061
Annu Rev Anal Chem (Palo Alto Calif). 2019 Jun 12;12(1):201-224
pubmed: 30848927
Anal Chem. 2011 Oct 15;83(20):7888-94
pubmed: 21882854
Rapid Commun Mass Spectrom. 2013 Dec 30;27(24):2745-50
pubmed: 24214859