Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver.

Animals Mice Liver / metabolism Hematopoietic Stem Cells / metabolism
Antibody Developmental biology Microscopy Stem Cells

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 12 2022
Historique:
received: 19 11 2021
revised: 27 04 2022
accepted: 28 06 2022
pubmed: 13 10 2022
medline: 22 12 2022
entrez: 12 10 2022
Statut: ppublish

Résumé

Understanding the murine fetal liver (FL) hematopoietic microenvironment, which promotes HSC proliferation, warrants identifying innate relationships between stem cells and the niche. An inclusive study of these cell associations remains elusive. Here, we optimized a protocol to immunolabel HSCs alongside the FL vasculature, a promising niche component. We provide a comprehensive plan from tissue processing, immunohistochemistry, and confocal microscopy, to three-dimensional distance analyses between HSCs and vasculature. This technique can be adapted for achieving congruous outcomes for other cell types. For complete details on the use and execution of this protocol, please refer to Biswas et al. (2020).

Identifiants

DOI: 10.1016/j.xpro.2022.101580 PMID: 36223268 PMC: PMC9576628
pubmed: 36223268
pii: S2666-1667(22)00460-9
doi: 10.1016/j.xpro.2022.101580
pmc: PMC9576628
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101580

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Atreyi Biswas (A)

Stem Cells and Development Lab, School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Maruthamala Campus, Vithura, Kerala 695551, India. Electronic address: atreyibiswas14@iisertvm.ac.in.

Shailendra Kumar Singh (SK)

Stem Cells and Development Lab, School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Maruthamala Campus, Vithura, Kerala 695551, India.

Gayathri M Kartha (GM)

Stem Cells and Development Lab, School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Maruthamala Campus, Vithura, Kerala 695551, India.

Satish Khurana (S)

Stem Cells and Development Lab, School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Maruthamala Campus, Vithura, Kerala 695551, India. Electronic address: satishkhurana@iisertvm.ac.in.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Immuno-localization of definitive...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Immuno-localization of definitive...
questionsmedicales.fr › Système digestif › Foie › Immuno-localization of definitive...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système hématopoïétique › Cellules de la moelle osseuse › Cellules souches hématopoïétiques › Immuno-localization of definitive...