Opsin knockdown specifically slows phototransduction in broadband and UV-sensitive photoreceptors in Periplaneta americana.
Periplaneta americana
Photoreceptor
Phototransduction
Quantum bump
Journal
Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
ISSN: 1432-1351
Titre abrégé: J Comp Physiol A Neuroethol Sens Neural Behav Physiol
Pays: Germany
ID NLM: 101141792
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
received:
24
05
2022
accepted:
27
09
2022
revised:
27
09
2022
pubmed:
13
10
2022
medline:
15
12
2022
entrez:
12
10
2022
Statut:
ppublish
Résumé
Photoreceptors with different spectral sensitivities serve different physiological and behavioral roles. We hypothesized that such functional evolutionary optimization could also include differences in phototransduction dynamics. We recorded elementary responses to light, quantum bumps (QBs), of broadband green-sensitive and ultraviolet (UV)-sensitive photoreceptors in the cockroach, Periplaneta americana, compound eyes using intracellular recordings. In addition to control photoreceptors, we used photoreceptors from cockroaches whose green opsin 1 (GO1) or UV opsin expression was suppressed by RNA interference. In the control broadband and UV-sensitive photoreceptors average input resistances were similar, but the membrane capacitance, a proxy for membrane area, was smaller in the broadband photoreceptors. QBs recorded in the broadband photoreceptors had comparatively short latencies, high amplitudes and short durations. Absolute sensitivities of both opsin knockdown photoreceptors were significantly lower than in wild type, and, unexpectedly, their latency was significantly longer while the amplitudes were not changed. Morphologic examination of GO1 knockdown photoreceptors did not find significant differences in rhabdom size compared to wild type. Our results differ from previous findings in Drosophila melanogaster rhodopsin mutants characterized by progressive rhabdomere degeneration, where QB amplitudes were larger but phototransduction latency was not changed compared to wild type.
Identifiants
pubmed: 36224473
doi: 10.1007/s00359-022-01580-z
pii: 10.1007/s00359-022-01580-z
doi:
Substances chimiques
Opsins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
591-604Subventions
Organisme : Russian National Foundation
ID : 22-24-00806
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : RGPIN-2021-02469
Organisme : Natural Sciences and Engineering Research Council of Canada
ID : RGPIN-2015-03712
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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