Sporozoite motility as a quantitative readout for anti-CSP antibody inhibition.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 10 2022
13 10 2022
Historique:
received:
08
07
2022
accepted:
10
10
2022
entrez:
13
10
2022
pubmed:
14
10
2022
medline:
18
10
2022
Statut:
epublish
Résumé
Antibodies can prevent malaria by neutralizing the infectious Plasmodium falciparum sporozoites (SPZ) before they establish an infection in the liver. Circumsporozoite protein (CSP), the most abundant surface protein of SPZ is the leading candidate for passive (and subunit) immunization approaches against malaria. Comprehensive assessment of the parasite-inhibitory capacity of anti-CSP monoclonal antibodies (mAbs) is an important step in advancing CSP-based immunization strategies. In this study, we employed a quantitative imaging-based motility assay to quantify the effect of anti-CSP mAbs on SPZ motility, both in vitro and in human skin.Our assay provided a quantitative measure of mAb parasite-inhibitory capacity through measurement of the half-maximal motility inhibitory concentration (IC
Identifiants
pubmed: 36229488
doi: 10.1038/s41598-022-22154-8
pii: 10.1038/s41598-022-22154-8
pmc: PMC9561690
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Protozoan
0
Malaria Vaccines
0
Membrane Proteins
0
Protozoan Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
17194Informations de copyright
© 2022. The Author(s).
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