Algal photosystem I dimer and high-resolution model of PSI-plastocyanin complex.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
05
09
2021
accepted:
02
09
2022
pubmed:
14
10
2022
medline:
21
10
2022
entrez:
13
10
2022
Statut:
ppublish
Résumé
Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Cryogenic electron microscopy identified that the absence of PsaH and Lhca2 gives rise to a head-to-head relative orientation of the PSI-light-harvesting complex I monomers in a way that is essentially different from the oligomer formation in cyanobacteria. The light-harvesting protein Lhca9 is the key element for mediating this dimerization. The interface between the monomers is lacking PsaH and thus partially overlaps with the surface area that would bind one of the light-harvesting complex II complexes in state transitions. We also define the most accurate available PSI-light-harvesting complex I model at 2.3 Å resolution, including a flexibly bound electron donor plastocyanin, and assign correct identities and orientations to all the pigments, as well as 621 water molecules that affect energy transfer pathways.
Identifiants
pubmed: 36229605
doi: 10.1038/s41477-022-01253-4
pii: 10.1038/s41477-022-01253-4
pmc: PMC9579051
doi:
Substances chimiques
Photosystem I Protein Complex
0
Plastocyanin
9014-09-9
Light-Harvesting Protein Complexes
0
Protein Subunits
0
Water
059QF0KO0R
Photosystem II Protein Complex
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1191-1201Informations de copyright
© 2022. The Author(s).
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