HIV-1 Infection of Long-Lived Hematopoietic Precursors In Vitro and In Vivo.
CXCR4 and CCR5 tropism
HIV-1 infection
colony formation
hematopoietic stem and progenitor cells (HSPCs)
hematopoietic stem cells (HSC)
in vitro and in vivo
multipotent progenitor cells (MPP)
proviral DNA
replating assays
viral reservoir
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
23 09 2022
23 09 2022
Historique:
received:
09
08
2022
revised:
13
09
2022
accepted:
19
09
2022
entrez:
14
10
2022
pubmed:
15
10
2022
medline:
18
10
2022
Statut:
epublish
Résumé
Latent reservoirs in human-immunodeficiency-virus-1 (HIV-1)-infected individuals represent a major obstacle in finding a cure for HIV-1. Hematopoietic stem and progenitor cells (HSPCs) have been described as potential HIV-1 targets, but their roles as HIV-1 reservoirs remain controversial. Here we provide additional evidence for the susceptibility of several distinct HSPC subpopulations to HIV-1 infection in vitro and in vivo. In vitro infection experiments of HSPCs were performed with different HIV-1 Env-pseudotyped lentiviral particles and with replication-competent HIV-1. Low-level infection/transduction of HSPCs, including hematopoietic stem cells (HSCs) and multipotent progenitors (MPP), was observed, preferentially via CXCR4, but also via CCR5-mediated entry. Multi-lineage colony formation in methylcellulose assays and repetitive replating of transduced cells provided functional proof of susceptibility of primitive HSPCs to HIV-1 infection. Further, the access to bone marrow samples from HIV-positive individuals facilitated the detection of HIV-1
Identifiants
pubmed: 36230931
pii: cells11192968
doi: 10.3390/cells11192968
pmc: PMC9562211
pii:
doi:
Substances chimiques
DNA, Complementary
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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