Electrochemical Biosensing of Glucose Based on the Enzymatic Reduction of Glucose.
aldose reductase
electrochemical glucose sensors
glucose reduction
Journal
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366
Informations de publication
Date de publication:
20 Sep 2022
20 Sep 2022
Historique:
received:
22
08
2022
revised:
10
09
2022
accepted:
14
09
2022
entrez:
14
10
2022
pubmed:
15
10
2022
medline:
18
10
2022
Statut:
epublish
Résumé
In this work, the enzyme aldehyde reductase, also known as aldose reductase, was synthesized and cloned from a human gene. Spectrophotometric measurements show that in presence of the nicotinamide adenine dinucleotide phosphate cofactor (NADPH), the aldehyde reductase catalyzed the reduction of glucose to sorbitol. Electrochemical measurements performed on an electrodeposited poly(methylene green)-modified gold electrode showed that in the presence of the enzyme aldehyde reductase, the electrocatalytic oxidation current of NADPH decreased drastically after the addition of glucose. These results demonstrate that aldehyde reductase is an enzyme that allows the construction of an efficient electrochemical glucose biosensor based on glucose reduction.
Identifiants
pubmed: 36236202
pii: s22197105
doi: 10.3390/s22197105
pmc: PMC9572614
pii:
doi:
Substances chimiques
Sorbitol
506T60A25R
NADP
53-59-8
Gold
7440-57-5
Aldehyde Reductase
EC 1.1.1.21
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-15-CE05-0003-01
Organisme : Agence Nationale de la Recherche
ID : ANR-16-CE19-0007-03
Organisme : The Engineering and Physical Sciences Research Council
ID : EPSRC
Références
Drug Metab Rev. 2008;40(4):553-624
pubmed: 18949601
Sensors (Basel). 2010;10(5):4558-76
pubmed: 22399892
Biomacromolecules. 2004 Jul-Aug;5(4):1241-7
pubmed: 15244436
Free Radic Biol Med. 2011 Mar 1;50(5):567-75
pubmed: 21163346
ACS Appl Mater Interfaces. 2011 Aug;3(8):3049-57
pubmed: 21736289
Colloids Surf B Biointerfaces. 2020 Apr 21;192:111065
pubmed: 32361503
Biochim Biophys Acta. 1960 Jan 1;37:120-6
pubmed: 14401390
Endocr Rev. 2019 Apr 1;40(2):447-475
pubmed: 30137266
Chem Rev. 2008 Feb;108(2):423-61
pubmed: 18229952
Biosensors (Basel). 2022 Jun 24;12(7):
pubmed: 35884254
PLoS One. 2021 Aug 9;16(8):e0255484
pubmed: 34370769
Clin Chim Acta. 2009 Apr;402(1-2):7-13
pubmed: 19167374
Biosens Bioelectron. 2005 Jun 15;20(12):2435-53
pubmed: 15854818
Analyst. 2006 Feb;131(2):257-61
pubmed: 16440091
Chem Soc Rev. 2020 Nov 7;49(21):7671-7709
pubmed: 33020790
Chem Rev. 2008 Feb;108(2):814-25
pubmed: 18154363
Sensors (Basel). 2021 Jul 08;21(14):
pubmed: 34300412
J Food Drug Anal. 2015 Jun;23(2):191-200
pubmed: 28911373
Front Chem. 2021 Jul 29;9:723186
pubmed: 34395386
Nanoscale Adv. 2022 May 4;4(11):2420-2427
pubmed: 35746900
Crit Care Med. 2001 May;29(5):1062-70
pubmed: 11378622
Talanta. 2021 Jul 1;229:122275
pubmed: 33838777
Biosens Bioelectron. 2018 Mar 15;101:317-321
pubmed: 29033058
Chem Sci. 2015 Aug 1;6(8):4867-4875
pubmed: 28717492