A-MYB/TCFL5 regulatory architecture ensures the production of pachytene piRNAs in placental mammals.
A-MYB
TCFL5
pachytene piRNAs
spermatogenesis
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
14 Oct 2022
14 Oct 2022
Historique:
received:
06
10
2022
accepted:
07
10
2022
entrez:
14
10
2022
pubmed:
15
10
2022
medline:
15
10
2022
Statut:
aheadofprint
Résumé
In male mice, the transcription factor A MYB initiates the transcription of pachytene piRNA genes during meiosis. Here, we report that A MYB activates the transcription factor Tcfl5 produced in pachytene spermatocytes. Subsequently, A MYB and TCFL5 reciprocally reinforce their own transcription to establish a positive feedback circuit that triggers pachytene piRNA production. TCFL5 regulates the expression of genes required for piRNA maturation and promotes transcription of evolutionarily young pachytene piRNA genes, whereas A-MYB activates the transcription of older pachytene piRNA genes. Intriguingly, pachytene piRNAs from TCFL5-dependent young loci initiates the production of piRNAs from A-MYB-dependent older loci ensuring the self-propagation of pachytene piRNAs. A MYB and TCFL5 act via a set of incoherent feedforward loops that drive regulation of gene expression by pachytene piRNAs during spermatogenesis. This regulatory architecture is conserved in rhesus macaque, suggesting that it was present in the last common ancestor of placental mammals.
Identifiants
pubmed: 36241367
pii: rna.079472.122
doi: 10.1261/rna.079472.122
pmc: PMC9808571
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NICHD NIH HHS
ID : P01 HD078253
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136275
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM062862
Pays : United States
Organisme : NIH HHS
ID : S10 OD028576
Pays : United States
Informations de copyright
Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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