Carbamoyltransferase Enzyme Assay:
5-carbamoyloxymethylcytosine
5-hydroxymethylcytosine
Carbamoyltransferase
DNA modification
RNA modification
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2022
05 Sep 2022
Historique:
received:
08
04
2022
revised:
07
07
2022
accepted:
13
07
2022
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
18
10
2022
Statut:
epublish
Résumé
Nucleic acids in living organisms are more complex than the simple combinations of the four canonical nucleotides. Recent advances in biomedical research have led to the discovery of numerous naturally occurring nucleotide modifications and enzymes responsible for the synthesis of such modifications. In turn, these enzymes can be leveraged towards toolkits for DNA and RNA manipulation for epigenetic sequencing or other biotechnological applications. Here, we present the protocol to obtain purified 5-hydroxymethylcytosine carbamoyltransferase enzymes and the associated assays to convert 5-hydroxymethylcytosine to 5-carbamoyloxymethylcytosine
Identifiants
pubmed: 36245800
doi: 10.21769/BioProtoc.4496
pii: e4496
pmc: PMC9510146
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests All authors are employees of New England Biolabs Inc., a manufacturer and vendor of molecular biology reagents, including several enzymes and buffers used in this work.
Références
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