Increased Corneal Endothelial Cell Migration in Fuchs Endothelial Corneal Dystrophy: A Live Cell Imaging Study.
CE, corneal endothelium
CEC, corneal endothelial cell
Cell migration
Corneal endothelium
DM, Descemet’s membrane
DMEK, Descemet's membrane endothelial keratoplasty
DWEK, descemetorhexis without endothelial keratoplasty
Descemetorhexis without endothelial keratoplasty
Descemet’s stripping only
ECD, endothelial cell density
ECM, extracellular matrix
EMT, endothelial-to-mesenchymal transition
FECD, Fuchs endothelial corneal dystrophy
Fuchs endothelial corneal dystrophy
GFP, green fluorescent protein
LNP, lipid nanoparticle
PBS, phosphate-buffered saline
TCF4, transcription factor 4
Journal
Ophthalmology science
ISSN: 2666-9145
Titre abrégé: Ophthalmol Sci
Pays: Netherlands
ID NLM: 9918230896206676
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
11
11
2020
revised:
09
02
2021
accepted:
03
03
2021
entrez:
17
10
2022
pubmed:
9
3
2021
medline:
9
3
2021
Statut:
epublish
Résumé
To investigate if corneal endothelial cells (CECs) in Fuchs endothelial corneal dystrophy (FECD) have altered cellular migration compared with normal controls. Comparative analysis. Descemet's membrane and CECs derived from patients with FECD undergoing endothelial keratoplasty or normal cadaveric donors. Ex vivo specimens were used for live cell imaging and generation of immortalized cell lines. Live imaging was performed on FECD and normal CECs and on ex vivo specimens transfected with green fluorescent protein. Migration speeds were determined as a function of cellular density using automated cell tracking. Ex vivo specimens were classified as either FECD or normal low cell density (nonconfluent) or high cell density (confluent). Scratch assay was performed on CECs seeded at high confluence to determine migration speed. Genetic analysis from blood samples or CECs was performed to detect a CTG repeat expansion in the Mean cell migration speed. Fuchs endothelial corneal dystrophy CECs in low cell density areas displayed increased mean speed (0.391 ± 0.005 μm/minute vs. 0.364 ± 0.005 μm/minute; Fuchs endothelial corneal dystrophy CECs demonstrated increased migration speed compared with normal CECs. Further investigation into the mechanisms of heightened cell migration in FECD is needed and may provide insight into its pathogenesis, as well as having implications on descemetorhexis without endothelial keratoplasty.
Identifiants
pubmed: 36246012
doi: 10.1016/j.xops.2021.100006
pii: S2666-9145(21)00004-X
pmc: PMC9559113
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100006Informations de copyright
© 2021 by the American Academy of Ophthalmology.
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