A matter of origin - identification of SEMA3A, BGLAP, SPP1 and PHEX as distinctive molecular features between bone site-specific human osteoblasts on transcription level.
3D-microchip culture
alveolar bone
bone grafts
gene expression analysis
iliac crest
in vitro model
osteoblasts
vitamin D
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2022
2022
Historique:
received:
12
04
2022
accepted:
12
09
2022
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
18
10
2022
Statut:
epublish
Résumé
In oral and maxillofacial bone reconstruction, autografts from the iliac crest represent the gold standard due to their superior clinical performance, compared to autografts derived from other extraoral regions. Thus, the aim of our study was to identify putative differences between osteoblasts derived from alveolar (hOB-A) and iliac crest (hOB-IC) bone of the same donor (nine donors) by means of their molecular properties in 2D and 3D culture. We thereby focused on the gene expression of biomarkers involved in osteogenic differentiation, matrix formation and osteoclast modulation. Furthermore, we examined the transcriptional response to Vit.D3 in hOB-A and hOB-IC. Our results revealed different modulation modes of the biomarker expression in osteoblasts, namely cell origin/bone entity-dependent, and culture configuration- and/or time-dependent modulations. SEMA3A, SPP1, BGLAP and PHEX demonstrated the strongest dependence on cell origin. With respect to Vit.D3-effects, BGLAP, SPP1 and ALPL displayed the highest Vit.D3-responsiveness. In this context we demonstrated that the transcriptional Vit.D3-response concerning SPP1 and ALPL in human osteoblasts depended on the cell origin. The results indicate a higher bone remodeling activity of iliac crest than alveolar osteoblasts and support the growing evidence that a high osteoclast activity at the host-/donor bone interface may support graft integration.
Identifiants
pubmed: 36246375
doi: 10.3389/fbioe.2022.918866
pii: 918866
pmc: PMC9554416
doi:
Types de publication
Journal Article
Langues
eng
Pagination
918866Informations de copyright
Copyright © 2022 Zhang, Rau, Kotzagiorgis, Rothweiler, Nahles, Gottwald, Rolauffs, Steinberg, Nelson and Altmann.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Phys Med. 2015 May;31(3):307-13
pubmed: 25726476
Clin Chem. 2020 Aug 1;66(8):1012-1029
pubmed: 32746458
Nature. 2012 May 3;485(7396):69-74
pubmed: 22522930
J Craniomaxillofac Surg. 2015 Jun;43(5):705-9
pubmed: 25937474
J Tissue Eng Regen Med. 2018 May;12(5):1238-1246
pubmed: 29489057
Eur J Oral Implantol. 2014 Summer;7 Suppl 2:S203-17
pubmed: 24977256
J Biomed Mater Res A. 2005 Jun 1;73(3):284-94
pubmed: 15800855
Mol Endocrinol. 2008 Jun;22(6):1370-81
pubmed: 18356290
Prog Orthod. 2018 Jun 25;19(1):18
pubmed: 29938297
J Cell Sci. 2011 Apr 1;124(Pt 7):991-8
pubmed: 21402872
J Bone Miner Metab. 2007;25(5):266-76
pubmed: 17704991
Biomaterials. 2011 Dec;32(34):8947-56
pubmed: 21868090
J Bone Miner Res. 2009 Nov;24(11):1927-35
pubmed: 19419324
Biochim Biophys Acta. 2009 Aug;1792(8):757-65
pubmed: 19465118
J Cell Physiol. 2018 Jul;233(7):5334-5347
pubmed: 29215722
Biomed Microdevices. 2006 Sep;8(3):191-9
pubmed: 16718404
Int J Mol Sci. 2019 Apr 04;20(7):
pubmed: 30987410
J Biol Chem. 2005 Dec 9;280(49):40589-98
pubmed: 16195230
Cell Differ. 1987 Sep;21(4):247-54
pubmed: 3304665
Dev Dyn. 2007 Jul;236(7):1876-90
pubmed: 17497678
J Steroid Biochem Mol Biol. 2004 May;89-90(1-5):269-71
pubmed: 15225783
Tissue Eng. 2005 Jan-Feb;11(1-2):182-91
pubmed: 15738673
J Craniomaxillofac Surg. 2015 Oct;43(8):1487-93
pubmed: 26315276
J Clin Pathol. 2008 May;61(5):577-87
pubmed: 18441154
BMC Musculoskelet Disord. 2018 May 24;19(1):168
pubmed: 29793458
Front Bioeng Biotechnol. 2019 Oct 10;7:251
pubmed: 31649925
Calcif Tissue Int. 2014 Jan;94(1):25-34
pubmed: 24002178
Clin Oral Investig. 2015 Jul;19(6):1223-33
pubmed: 25366872
Int J Mol Sci. 2020 Jul 17;21(14):
pubmed: 32709153
PLoS Genet. 2020 May 28;16(5):e1008586
pubmed: 32463816
Front Bioeng Biotechnol. 2020 Apr 24;8:336
pubmed: 32391343
Hum Mol Genet. 2019 Mar 15;28(6):896-911
pubmed: 30445456
PLoS Genet. 2020 Jun 2;16(6):e1008714
pubmed: 32484816
J Bone Miner Res. 2013 Mar;28(3):688-99
pubmed: 22991293
Clin Oral Implants Res. 2009 Mar;20(3):319-26
pubmed: 19397644
Sci Rep. 2015 Mar 10;5:8948
pubmed: 25752700
Oral Dis. 2008 Jul;14(5):465-71
pubmed: 18938273
J Bone Miner Res. 2011 Feb;26(2):229-38
pubmed: 21254230
Tissue Eng Part C Methods. 2013 Nov;19(11):850-63
pubmed: 23581275
Exp Oncol. 2004 Sep;26(3):179-84
pubmed: 15494684
Stem Cells Int. 2016;2016:9150819
pubmed: 27956902
Tissue Cell. 2018 Jun;52:9-16
pubmed: 29857833
Clin Oral Implants Res. 2015 Jul;26(7):747-52
pubmed: 24953889
Biotechnol Genet Eng Rev. 2010;26:163-78
pubmed: 21415880
Biomaterials. 2014 Mar;35(10):3208-19
pubmed: 24439401
Int J Mol Sci. 2018 Sep 23;19(10):
pubmed: 30249051
Stem Cell Res Ther. 2018 May 11;9(1):131
pubmed: 29751774
J Cell Biol. 1994 Nov;127(4):1149-58
pubmed: 7962073
Sci Rep. 2017 May 25;7(1):2409
pubmed: 28546538
J Bone Miner Metab. 2019 Jan;37(1):105-117
pubmed: 29327303
Nanomedicine (Lond). 2015;10(5):753-63
pubmed: 25816878
PLoS One. 2018 Sep 19;13(9):e0204171
pubmed: 30231062
Organogenesis. 2012 Oct-Dec;8(4):114-24
pubmed: 23247591
Front Physiol. 2014 Apr 09;5:137
pubmed: 24782782
PLoS One. 2015 Feb 27;10(2):e0118226
pubmed: 25723393
BMC Res Notes. 2011 Oct 14;4:410
pubmed: 21996334
Bone. 2016 May;86:10-21
pubmed: 26921824
Mol Cell Endocrinol. 2015 Sep 15;413:157-67
pubmed: 26112182
Microsc Res Tech. 1996 Feb 1;33(2):141-64
pubmed: 8845514
J Bone Miner Res. 2014 Nov;29(11):2336-45
pubmed: 24753124
Int J Biochem Cell Biol. 2016 Aug;77(Pt A):155-164
pubmed: 27270332
Nat Med. 1997 Feb;3(2):177-82
pubmed: 9018236
Curr Opin Otolaryngol Head Neck Surg. 2015 Aug;23(4):316-22
pubmed: 26101873