Pharmacological determination of the fractional block of Nav channels required to impair neuronal excitability and
epileptiform activity
inhibition
multiarray electrophysiology
pharmacology
sodium channel
tetrodotoxin
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2022
2022
Historique:
received:
09
06
2022
accepted:
31
08
2022
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
18
10
2022
Statut:
epublish
Résumé
Voltage-gated sodium channels (Nav) are essential for the initiation and propagation of action potentials in neurons. Of the nine human channel subtypes, Nav1.1, Nav1.2 and Nav1.6 are prominently expressed in the adult central nervous system (CNS). All three of these sodium channel subtypes are sensitive to block by the neurotoxin tetrodotoxin (TTX), with TTX being almost equipotent on all three subtypes. In the present study we have used TTX to determine the fractional block of Nav channels required to impair action potential firing in pyramidal neurons and reduce network seizure-like activity. Using automated patch-clamp electrophysiology, we first determined the IC
Identifiants
pubmed: 36246527
doi: 10.3389/fncel.2022.964691
pmc: PMC9557217
doi:
Types de publication
Journal Article
Langues
eng
Pagination
964691Informations de copyright
Copyright © 2022 Thouta, Waldbrook, Lin, Mahadevan, Mezeyova, Soriano, Versi, Goodchild and Parrish.
Déclaration de conflit d'intérêts
ST, MW, SL, AM, JM, MS, and SG are employees of Xenon Pharmaceuticals Inc. and may hold stock or stock options in the company. RP and PV were employees of Xenon Pharmaceuticals Inc.
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