Surface tension and viscosity of protein condensates quantified by micropipette aspiration.
Journal
Biophysical reports
ISSN: 2667-0747
Titre abrégé: Biophys Rep (N Y)
Pays: United States
ID NLM: 9918266001106676
Informations de publication
Date de publication:
08 Sep 2021
08 Sep 2021
Historique:
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
18
10
2022
Statut:
ppublish
Résumé
The material properties of biomolecular condensates have been suggested to play important biological and pathological roles. Despite the rapid increase in the number of biomolecules identified that undergo liquid-liquid phase separation, quantitative studies and direct measurements of the material properties of the resulting condensates have been severely lagging behind. Here, we develop a micropipette-based technique that uniquely, to our knowledge, allows quantifications of both the surface tension and viscosity of biomolecular condensates, independent of labeling and surface-wetting effects. We demonstrate the accuracy and versatility of this technique by measuring condensates of LAF-1 RGG domains and a polymer-based aqueous two-phase system. We further confirm our measurements using established condensate fusion and fluorescence recovery after photobleaching assays. We anticipate the micropipette-based technique will be widely applicable to biomolecular condensates and will resolve several limitations regarding current approaches.
Identifiants
pubmed: 36247368
doi: 10.1016/j.bpr.2021.100011
pmc: PMC9563586
mid: NIHMS1802015
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIDA NIH HHS
ID : R21 DA056322
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM135141
Pays : United States
Déclaration de conflit d'intérêts
DECLARATION OF INTERESTS The authors declare no competing interests.
Références
Science. 2017 Sep 22;357(6357):
pubmed: 28935776
Science. 2020 Oct 2;370(6512):56-60
pubmed: 33004511
Biophys J. 2019 Oct 1;117(7):1285-1300
pubmed: 31540706
Langmuir. 2020 May 19;36(19):5186-5191
pubmed: 32347734
Biophys J. 1999 Jan;76(1 Pt 1):110-28
pubmed: 9876128
Science. 2020 Dec 11;370(6522):1317-1323
pubmed: 33303613
Trends Cell Biol. 2019 Apr;29(4):308-322
pubmed: 30660504
Annu Rev Genet. 2019 Dec 3;53:171-194
pubmed: 31430179
Cell. 2018 Apr 19;173(3):677-692.e20
pubmed: 29677512
J Biomech. 2000 Jan;33(1):15-22
pubmed: 10609514
J Nanotechnol Eng Med. 2014 Nov;5(4):0408011-408016
pubmed: 26155329
Interface Focus. 2018 Oct 6;8(5):20180032
pubmed: 30443328
Nat Chem Biol. 2021 Jun;17(6):693-702
pubmed: 34035521
Mol Cell. 2015 Oct 15;60(2):220-30
pubmed: 26474065
Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7189-94
pubmed: 26015579
Nature. 2021 Mar;591(7848):142-146
pubmed: 33473217
Nat Rev Neurol. 2019 May;15(5):272-286
pubmed: 30890779
Nat Commun. 2018 Jul 30;9(1):2985
pubmed: 30061688
Biophys J. 2021 Apr 6;120(7):1161-1169
pubmed: 33453268
Nat Biotechnol. 2019 Dec;37(12):1435-1445
pubmed: 31792412
Angew Chem Int Ed Engl. 2020 Nov 16;59(47):20837-20840
pubmed: 32767698
Science. 2016 Apr 29;352(6285):595-9
pubmed: 27056844
Science. 2018 Aug 10;361(6402):604-607
pubmed: 29976799
Nat Rev Mol Cell Biol. 2017 May;18(5):285-298
pubmed: 28225081
Elife. 2016 May 24;5:
pubmed: 27215841
J Am Chem Soc. 2002 Nov 13;124(45):13374-5
pubmed: 12418876
Langmuir. 2012 Feb 28;28(8):3831-9
pubmed: 22292882
Nat Struct Mol Biol. 2019 Mar;26(3):220-226
pubmed: 30833787
Science. 1999 May 14;284(5417):1143-6
pubmed: 10325219
J Phys Chem B. 2021 Apr 15;125(14):3441-3451
pubmed: 33661634
Phys Rev E. 2021 Mar;103(3-1):032409
pubmed: 33862816
Nat Commun. 2015 Jan 08;6:5974
pubmed: 25569184
Science. 2009 Jun 26;324(5935):1729-32
pubmed: 19460965
Nat Phys. 2021 Apr;17(4):493-498
pubmed: 35211183
Science. 2021 Feb 5;371(6529):
pubmed: 33335017
Cell. 2019 Jan 24;176(3):419-434
pubmed: 30682370
J Am Chem Soc. 2016 Aug 17;138(32):10157-62
pubmed: 27433782
Phys Rev Lett. 2018 Dec 21;121(25):258101
pubmed: 30608810
Nat Commun. 2020 Sep 15;11(1):4628
pubmed: 32934220
Phys Rev Lett. 1990 Apr 23;64(17):2094-2097
pubmed: 10041575
J Mol Biol. 2021 Jun 11;433(12):166961
pubmed: 33774037
Langmuir. 2014 Aug 19;30(32):9691-9
pubmed: 25068649
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11421-11431
pubmed: 32393642
Phys Rev Lett. 2018 Oct 5;121(14):148101
pubmed: 30339413
Cell. 2016 Jun 16;165(7):1686-1697
pubmed: 27212236
Soft Matter. 2016 Nov 16;12(45):9142-9150
pubmed: 27791212