Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.
Meiosis
Spermatocytes
Spermatogenesis
Spermatogonia
Testis
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
15 11 2022
15 11 2022
Historique:
received:
02
03
2022
accepted:
10
10
2022
pubmed:
18
10
2022
medline:
19
11
2022
entrez:
17
10
2022
Statut:
ppublish
Résumé
In mammalian testes, premeiotic spermatogonia respond to retinoic acid by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due to two unresolved technical challenges: (1) lack of robust and reliable in vitro models to study differentiation and meiotic initiation; and (2) lack of methods to isolate large and pure populations of male germ cells at each stage of differentiation and at meiotic initiation. Here, we report a facile in vitro differentiation and meiotic initiation system that can be readily manipulated, including the use of chemical agents that cannot be safely administered to live animals. In addition, we present a transgenic mouse model enabling fluorescence-activated cell sorting-based isolation of millions of spermatogonia at specific developmental stages as well as meiotic spermatocytes.
Identifiants
pubmed: 36250451
pii: 282465
doi: 10.1242/dev.200713
pmc: PMC9845750
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NICHD NIH HHS
ID : R21 HD105963
Pays : United States
Organisme : NIH HHS
ID : S10 OD021615
Pays : United States
Organisme : NIH HHS
ID : S10-OD021615
Pays : United States
Organisme : NIH HHS
ID : HD090083
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD090007
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD090083
Pays : United States
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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