Comparison of nucleocapsid and spike antibody ELISAs for determining SARS-CoV-2 seropositivity in Kenyan women and infants.
ELISA
Kenya
SARS-CoV-2
nucleocapsid
serology
spike
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
revised:
23
09
2022
received:
01
08
2022
accepted:
03
10
2022
pubmed:
18
10
2022
medline:
11
1
2023
entrez:
17
10
2022
Statut:
ppublish
Résumé
A multitude of enzyme-linked immunosorbent assays (ELISAs) has been developed to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies since the coronavirus disease 2019 pandemic started in late 2019. Assessing the reliability of these assays in diverse global populations is critical. This study compares the use of the commercially available Platelia Total Ab Assay (Bio-Rad) nucleocapsid ELISA to the widely used Mount Sinai spike IgG ELISA in a Kenyan population seroprevalence study. Using longitudinal plasma specimens collected from a mother-infant cohort living in Nairobi, Kenya between May 2019 and December 2020, this study demonstrates that the two assays have a high qualitative agreement (92.7%) and strong correlation of antibody levels (R
Identifiants
pubmed: 36251533
doi: 10.1002/jmv.28221
pmc: PMC9839577
mid: NIHMS1843372
doi:
Substances chimiques
Antibodies, Viral
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e28221Subventions
Organisme : CIHR
Pays : Canada
Organisme : NICHD NIH HHS
ID : R01 HD092311
Pays : United States
Organisme : NIDDK NIH HHS
ID : R00 DK107923
Pays : United States
Organisme : NIH HHS
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI138709
Pays : United States
Organisme : CIHR
ID : 202005
Pays : Canada
Organisme : CIHR
ID : 201709
Pays : Canada
Informations de copyright
© 2022 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.
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