No evidence for intervention-associated DNA methylation changes in monocytes of patients with posttraumatic stress disorder.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 10 2022
17 10 2022
Historique:
received:
03
08
2021
accepted:
11
10
2022
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
20
10
2022
Statut:
epublish
Résumé
DNA methylation patterns can be responsive to environmental influences. This observation has sparked interest in the potential for psychological interventions to influence epigenetic processes. Recent studies have observed correlations between DNA methylation changes and therapy outcome. However, most did not control for changes in cell composition. This study had two aims: first, we sought to replicate therapy-associated changes in DNA methylation of commonly assessed candidate genes in isolated monocytes from 60 female patients with post-traumatic stress disorder (PTSD). Our second, exploratory goal was to identify novel genomic regions with substantial pre-to-post intervention DNA methylation changes by performing whole-genome bisulfite sequencing (WGBS) in two patients with PTSD. Equivalence testing and Bayesian analyses provided evidence against physiologically meaningful intervention-associated DNA methylation changes in monocytes of PTSD patients in commonly investigated target genes (NR3C1, FKBP5, SLC6A4, OXTR). Furthermore, WGBS yielded only a limited set of candidate regions with suggestive evidence of differential DNA methylation pre- to post-therapy. These differential DNA methylation patterns did not prove replicable when investigated in the entire cohort. We conclude that there is no evidence for major, recurrent intervention-associated DNA methylation changes in the investigated genes in monocytes of patients with PTSD.
Identifiants
pubmed: 36253434
doi: 10.1038/s41598-022-22177-1
pii: 10.1038/s41598-022-22177-1
pmc: PMC9576776
doi:
Substances chimiques
SLC6A4 protein, human
0
Serotonin Plasma Membrane Transport Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17347Informations de copyright
© 2022. The Author(s).
Références
Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20578-83
pubmed: 23184974
Epigenomics. 2017 Aug;9(8):1143-1150
pubmed: 28749184
Soc Psychol Personal Sci. 2017 May;8(4):355-362
pubmed: 28736600
Mol Psychiatry. 2006 Mar;11(3):224-6
pubmed: 16402131
Nat Methods. 2020 Mar;17(3):261-272
pubmed: 32015543
Psychol Psychother. 2019 Jun;92(2):190-207
pubmed: 30924323
Transl Psychiatry. 2020 Aug 24;10(1):296
pubmed: 32839428
Clin Epigenetics. 2016 Sep 06;8:92
pubmed: 27602172
Nat Neurosci. 2004 Aug;7(8):847-54
pubmed: 15220929
Clin Epigenetics. 2020 May 6;12(1):61
pubmed: 32375885
Bioinformatics. 2015 Jun 15;31(12):2032-4
pubmed: 25697820
Epigenetics Chromatin. 2015 May 09;8:19
pubmed: 25977707
Nat Rev Genet. 2009 Nov;10(11):805-11
pubmed: 19789556
Transl Psychiatry. 2013 Jan 15;3:e207
pubmed: 23422958
Genome Res. 2016 Feb;26(2):256-62
pubmed: 26631489
Am J Hum Biol. 2016 Sep 10;28(5):721-8
pubmed: 27088641
Bioinformatics. 2016 Jan 15;32(2):292-4
pubmed: 26428292
Circ Cardiovasc Genet. 2016 Oct;9(5):436-447
pubmed: 27651444
Depress Anxiety. 2015 Dec;32(12):861-70
pubmed: 26647360
Genome Biol Evol. 2014 Jun 12;6(7):1579-88
pubmed: 24923327
Nat Rev Genet. 2016 Aug;17(8):487-500
pubmed: 27346641
Psychoneuroendocrinology. 2020 Oct;120:104784
pubmed: 32673938
Transl Psychiatry. 2014 Sep 16;4:e444
pubmed: 25226553
J Dev Orig Health Dis. 2015 Oct;6(5):399-406
pubmed: 26017068
Mol Psychiatry. 2021 Apr;26(4):1264-1271
pubmed: 31645664
Gigascience. 2021 Feb 16;10(2):
pubmed: 33590861
Epigenetics Chromatin. 2016 Jul 29;9:33
pubmed: 27478504
Hum Mol Genet. 2005 Apr 15;14 Spec No 1:R47-58
pubmed: 15809273
Neuropsychopharmacology. 2018 Jan;43(2):342-353
pubmed: 28540928
Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16574-9
pubmed: 24062448
Mol Psychiatry. 2017 May;22(5):640-646
pubmed: 28289275
Nat Neurosci. 2013 Jan;16(1):33-41
pubmed: 23201972
Annu Rev Psychol. 2018 Jan 4;69:459-485
pubmed: 29035689
Dis Markers. 2011;30(2-3):123-32
pubmed: 21508516
FASEB J. 2010 Sep;24(9):3135-44
pubmed: 20385621
Mol Cell Neurosci. 2013 Mar;53:52-62
pubmed: 23064447
Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):4830-5
pubmed: 27078100
Psychoneuroendocrinology. 2010 Aug;35(7):955-62
pubmed: 20630660
Am J Med Genet B Neuropsychiatr Genet. 2019 Mar;180(2):150-158
pubmed: 30334356
Nat Rev Immunol. 2016 Jan;16(1):22-34
pubmed: 26711676
Behav Res Methods. 2007 May;39(2):175-91
pubmed: 17695343
Front Psychiatry. 2013 Sep 27;4:118
pubmed: 24098286
Nat Methods. 2015 Mar;12(3):230-2, 1 p following 232
pubmed: 25362363
Nat Methods. 2013 Oct;10(10):949-55
pubmed: 24076989
Epigenetics. 2010 Aug 16;5(6):516-26
pubmed: 20505345
Transl Psychiatry. 2016 Apr 05;6:e773
pubmed: 27045843
Genome Biol. 2016 Jun 27;17(1):138
pubmed: 27350042
Front Neurosci. 2015 Jan 21;8:447
pubmed: 25653581
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):3080-5
pubmed: 21300872
Sci Adv. 2018 Jan 31;4(1):eaao4364
pubmed: 29399631
Neurobiol Dis. 2010 Jul;39(1):66-72
pubmed: 20053376
Transl Psychiatry. 2019 Jan 15;9(1):1
pubmed: 30664621
Epigenetics. 2011 Aug;6(8):971-8
pubmed: 21772123
Transl Psychiatry. 2019 Sep 2;9(1):215
pubmed: 31477685