Fold-change of chromatin condensation in yeast is a conserved property.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 10 2022
17 10 2022
Historique:
received:
04
05
2022
accepted:
13
10
2022
entrez:
17
10
2022
pubmed:
18
10
2022
medline:
20
10
2022
Statut:
epublish
Résumé
During mitosis, chromatin is condensed and organized into mitotic chromosomes. Condensation is critical for genome stability and dynamics, yet the degree of condensation is significantly different between multicellular and single-cell eukaryotes. What is less clear is whether there is a minimum degree of chromosome condensation in unicellular eukaryotes. Here, we exploited two-photon microscopy to analyze chromatin condensation in live and fixed cells, enabling studies of some organisms that are not readily amenable to genetic modification. This includes the yeasts Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, and Candida albicans, as well as a protist Trypanosoma brucei. We found that mitotic chromosomes in this range of species are condensed about 1.5-fold relative to interphase chromatin. In addition, we used two-photon microscopy to reveal that chromatin reorganization in interphase human hepatoma cells infected by the hepatitis C virus is decondensed compared to uninfected cells, which correlates with the previously reported viral-induced changes in chromatin dynamics. This work demonstrates the power of two-photon microscopy to analyze chromatin in a broad range of cell types and conditions, including non-model single-cell eukaryotes. We suggest that similar condensation levels are an evolutionarily conserved property in unicellular eukaryotes and important for proper chromosome segregation. Furthermore, this provides new insights into the process of chromatin condensation during mitosis in unicellular organisms as well as the response of human cells to viral infection.
Identifiants
pubmed: 36253460
doi: 10.1038/s41598-022-22340-8
pii: 10.1038/s41598-022-22340-8
pmc: PMC9576780
doi:
Substances chimiques
Chromatin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17393Informations de copyright
© 2022. The Author(s).
Références
Elife. 2016 May 18;5:
pubmed: 27192037
Annu Rev Cell Dev Biol. 2008;24:105-29
pubmed: 18616427
Life Sci Alliance. 2021 Jun 3;4(7):
pubmed: 34083394
Curr Genet. 2019 Apr;65(2):407-415
pubmed: 30361853
Nucleic Acids Res. 1998 Oct 15;26(20):4626-34
pubmed: 9753730
EMBO J. 2007 Feb 21;26(4):1024-34
pubmed: 17268547
PLoS Negl Trop Dis. 2010 Apr 13;4(4):e658
pubmed: 20404998
J Cell Sci. 2021 Jan 8;134(1):
pubmed: 33419949
Mol Biol Cell. 2000 Apr;11(4):1293-304
pubmed: 10749930
PLoS Genet. 2019 Jun 19;15(6):e1008181
pubmed: 31216276
Mol Biol Cell. 2007 Feb;18(2):557-68
pubmed: 17151360
Appl Opt. 1971 Oct 1;10(10):2350-3
pubmed: 20111328
Curr Opin Cell Biol. 2016 Jun;40:15-22
pubmed: 26895139
J Cell Biol. 2002 Mar 4;156(5):805-15
pubmed: 11864994
PLoS Genet. 2015 Mar 06;11(3):e1005036
pubmed: 25748820
PLoS One. 2011;6(11):e28237
pubmed: 22140560
EMBO Rep. 2020 Feb 5;21(2):e48211
pubmed: 31886609
Life Sci Alliance. 2021 Mar 26;4(6):
pubmed: 33771877
Cell. 1997 May 16;89(4):511-21
pubmed: 9160743
Cell. 1997 Oct 3;91(1):35-45
pubmed: 9335333
Curr Genet. 2021 Jun;67(3):447-459
pubmed: 33404730
J Cell Biol. 1994 May;125(3):517-30
pubmed: 8175878
Elife. 2015 Nov 28;4:e1039
pubmed: 26615018
Genes Dev. 1998 Jul 1;12(13):1986-97
pubmed: 9649503
Science. 2018 Apr 6;360(6384):102-105
pubmed: 29472443
J Cell Biol. 1967 Mar;32(3):585-603
pubmed: 4166504
Mol Cell Biol. 2013 Mar;33(5):984-98
pubmed: 23263988
Sci Rep. 2019 Jun 20;9(1):8929
pubmed: 31222142
Curr Genet. 2018 Feb;64(1):303-316
pubmed: 28597304
Nucleic Acids Res. 2019 Mar 18;47(5):2455-2471
pubmed: 30698808
Curr Genet. 2020 Apr;66(2):437-443
pubmed: 31535185
Mol Cell Biol. 2011 Mar;31(5):1012-21
pubmed: 21173163