Proanthocyanidin biosynthesis in the developing wheat seed coat investigated by chemical and RNA-Seq analysis.

RNA‐Seq flavonoids gene function grain color proanthocyanidin wheat

Journal

Plant direct
ISSN: 2475-4455
Titre abrégé: Plant Direct
Pays: England
ID NLM: 101716131

Informations de publication

Date de publication:
Oct 2022
Historique:
received: 26 07 2022
revised: 08 09 2022
accepted: 13 09 2022
entrez: 18 10 2022
pubmed: 19 10 2022
medline: 19 10 2022
Statut: epublish

Résumé

The composition of proanthocyanidins in the testa (seed coat) of bread wheat was analyzed by thiolysis of PA oligomers from developing grain and found to consist of (+)-catechin monomers, with a small amount of (+)-gallocatechin. The average chain length of soluble PA stayed relatively constant between 10 and 20 days post-anthesis, whereas that of unextractable PA increased over the same period, suggesting that increases in chain length might account for the insolubility of PAs from mature wheat grain. We carried out RNA-Seq followed by differential expression analysis from dissected tissues of developing grain from red- and white-grained near-isogenic lines differing in the presence of an active

Identifiants

pubmed: 36254336
doi: 10.1002/pld3.453
pii: PLD3453
pmc: PMC9554643
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e453

Informations de copyright

© 2022 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.

Déclaration de conflit d'intérêts

The authors did not report any conflicts of interest.

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Auteurs

Simon P Vaughan (SP)

Rothamsted Research Harpenden UK.

John M Baker (JM)

Rothamsted Research Harpenden UK.

Lucia F Primavesi (LF)

Rothamsted Research Harpenden UK.

Archana Patil (A)

Rothamsted Research Harpenden UK.

Robert King (R)

Rothamsted Research Harpenden UK.

Keywan Hassani-Pak (K)

Rothamsted Research Harpenden UK.

Satish Kulasekaran (S)

Rothamsted Research Harpenden UK.

Jane Coghill (J)

School of Biological Sciences University of Bristol Bristol UK.

Jane L Ward (JL)

Rothamsted Research Harpenden UK.

Alison K Huttly (AK)

Rothamsted Research Harpenden UK.

Andrew L Phillips (AL)

Rothamsted Research Harpenden UK.

Classifications MeSH