Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) Stress.

Humans Histone Deacetylase Inhibitors / pharmacology Endoplasmic Reticulum Stress / physiology Unfolded Protein Response Endoplasmic Reticulum / metabolism RNA, Messenger / metabolism
Electron microscopy Endoplasmic reticulum (ER) stress Fluorescence microscopy HDAC inhibitor HDACi Unfolded protein response (UPR) Western blot qPCR

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 18 10 2022
pubmed: 19 10 2022
medline: 21 10 2022
Statut: ppublish

Résumé

The endoplasmic reticulum (ER) is a multifunctional cell organelle which is important for the folding and processing of proteins. Different endogenous and exogenous factors can disturb the ER homeostasis, causing ER stress and activating the unfolded protein response (UPR) to remove misfolded proteins and aggregates. ER stress and the UPR are associated with several human diseases, such as diabetes, Alzheimer's or Parkinson's disease, and cancer. Histone deacetylase inhibitors (HDACi) are used to treat cancer and were shown to induce ER stress/to modulate the UPR, although the exact mechanism is not fully understood and needs further research. Several approaches to monitoring ER stress exist. Here we describe methods including qPCR, Western blot, transmission electron microscopy, and fluorescence microscopy to analyze changes in mRNA and protein expression levels as well as defects in ER structures after HDAC inhibitor-induced ER stress.

Identifiants

DOI: 10.1007/978-1-0716-2788-4_17 PMID: 36255630
pubmed: 36255630
doi: 10.1007/978-1-0716-2788-4_17
doi:

Substances chimiques

Histone Deacetylase Inhibitors 0
RNA, Messenger 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

253-268

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Melisa Halilovic (M)

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Jena, Germany.

Lisa Marx-Blümel (L)

Department of Pediatric Hematology and Oncology, Children's Clinic, Jena University Hospital, Jena, Germany.
Research Center Lobeda, Jena University Hospital, Jena, Germany.

Christian Marx (C)

Center for Pandemic Vaccines and Therapeutics (ZEPAI), Paul Ehrlich Institute (PEI), Langen, Germany.

Katrin Buder (K)

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Jena, Germany.

James F Beck (JF)

Department of Pediatric Hematology and Oncology, Children's Clinic, Jena University Hospital, Jena, Germany.

Jürgen Sonnemann (J)

Department of Pediatric Hematology and Oncology, Children's Clinic, Jena University Hospital, Jena, Germany. juergen.sonnemann@med.uni-jena.de.
Research Center Lobeda, Jena University Hospital, Jena, Germany. juergen.sonnemann@med.uni-jena.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Assessment of HDAC Inhibitor-Induced...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Mécanismes moléculaires de l'action pharmacologique › Antienzymes › Inhibiteurs de désacétylase d'histone › Assessment of HDAC Inhibitor-Induced...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Stress du réticulum endoplasmique › Assessment of HDAC Inhibitor-Induced...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Réponse aux protéines mal repliées › Assessment of HDAC Inhibitor-Induced...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Réticulum endoplasmique › Assessment of HDAC Inhibitor-Induced...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Assessment of HDAC Inhibitor-Induced...